Dadmanesh Maryam, Ranjbar Mohammad Mehdi, Alavian Seyed Moayed, Ghorban Khodayar
Department of Infectious Diseases, School of Medicine, AJA University of Medical Sciences, Tehran, IR Iran.
Department of Immunology, School of Medicine, AJA University of Medical Sciences, Tehran, IR Iran.
Hepat Mon. 2015 Mar 5;15(3):e24173. doi: 10.5812/hepatmon.24173. eCollection 2015 Mar.
Hepatitis viruses are one of the most important concerns in the patients on hemodialysis who are at high risk for blood-borne infections. GB virus C (GBV-C)/hepatitis G virus (HGV) is positive-stranded RNA virus with global distribution, which codes structural and nonstructural proteins (such as NS3).
Review on literature about Iranian HGV isolates showed that there was no report on sequencing and phylogenetic characteristics of NS3 gene and therefore, this study attempted to answers these challenges.
Briefly, steps followed were as follows: preparation of nucleic acids from three Iranian hemodialysis HGV-positive samples and RNA extraction; reverse transcriptase-PCR (RT-PCR) for amplification of NS3 gene and gel electrophoresis; sequencing and analyzing sequencing data; NCBI Registering; blasting and alignment of sequences; and finally constructing the phylogenetic tree.
Nucleotide BLAST results emphasized that most similar sequences to Ir NS3 sequences were those from Europe (Spain, Germany, and the United Kingdom) and the United states and IR sequences were located with five leaves in a branch. In addition, homology of Ir sequences showed96.4% (between IR1 and IR2), 94.9% identity (between IR1 and IR3), and 96.5% identity (between IR2 and IR3).Furthermore, highly variation and significance differences in NS3 between GBC isolates from geographical regions and some little changes in IR sequences were seen. Finally, phylogenetic tree revealed that NS3 genotype of Iranian isolates was probably similar to European countries and USA.
Overall, results of present study were consistent with the data reported earlier base on 5' NTR in Iranian isolates and revealed genotype IR2 is major genotype in Iranian HGV-positive patient. It means there are higher similarities between Iranian and Europe-USA in HGV NS3 gene.
肝炎病毒是血液透析患者最重要的关注问题之一，这些患者面临血源性感染的高风险。GB病毒C（GBV-C）/庚型肝炎病毒（HGV）是一种正链RNA病毒，全球分布，编码结构和非结构蛋白（如NS3）。
对有关伊朗HGV分离株的文献进行综述表明，尚无关于NS3基因测序和系统发育特征的报道，因此，本研究试图应对这些挑战。
简要来说，遵循的步骤如下：从三份伊朗血液透析HGV阳性样本中制备核酸并进行RNA提取；逆转录聚合酶链反应（RT-PCR）扩增NS3基因并进行凝胶电泳；测序并分析测序数据；在美国国立生物技术信息中心（NCBI）注册；对序列进行比对和排列；最后构建系统发育树。
核苷酸BLAST结果强调，与伊朗NS3序列最相似的序列来自欧洲（西班牙、德国和英国）和美国，伊朗序列位于一个分支的五片叶子处。此外，伊朗序列之间的同源性显示为96.4%（IR1和IR2之间）、94.9%（IR1和IR3之间）和96.5%（IR2和IR3之间）。此外，在来自不同地理区域的GBV-C分离株的NS3之间观察到高度变异和显著差异，而伊朗序列有一些微小变化。最后，系统发育树显示伊朗分离株的NS3基因型可能与欧洲国家和美国相似。
总体而言，本研究结果与先前报道的基于伊朗分离株5'非翻译区（NTR）的数据一致，表明基因型IR2是伊朗HGV阳性患者中的主要基因型。这意味着伊朗与欧美在HGV NS3基因上有更高的相似性。
