Korean Medicine R&D Center, Dongguk University, Gyeongju 780-714, Republic of Korea.
Korean Medicine R&D Center, Dongguk University, Gyeongju 780-714, Republic of Korea; Department of Herbology, College of Korean Medicine, Dongguk University, Gyeongju 780-714, Republic of Korea.
Eur J Pharmacol. 2015 Jul 5;758:60-3. doi: 10.1016/j.ejphar.2015.03.059. Epub 2015 Mar 31.
Insulin resistance in skeletal muscle is a major risk factor for the development of type 2 diabetes (T2D). In this study, we investigated the effect of icariin on insulin resistance in C2C12 mouse skeletal muscle cells. C2C12 myoblasts were differentiated into myotubes for five days, then treated with icariin (50 and 100µM) or metformin (1mM) in the presence of 100nM insulin for 24h. Adiponectin production was measured in culture media by ELISA, and AMP-activated protein kinase (AMPK)/insulin signaling pathway activation was assessed by the western blot analysis. Icariin significantly increased adiponectin production in C2C12 myotubes. Moreover, icariin markedly promoted the phosphorylation of AMPK and acetyl-CoA carboxylase (ACC). Icariin up-regulated the expression of phosphatidylinositol 3-kinase (PI3K) and the phosphorylation of insulin receptor substrate-1 (IRS-1) in C2C12 myotubes. These results suggest that icariin has therapeutic potential for the treatment of T2D via the regulation of insulin resistance in skeletal muscle.
骨骼肌胰岛素抵抗是 2 型糖尿病 (T2D) 发生的主要危险因素。在本研究中，我们研究了淫羊藿苷对 C2C12 小鼠骨骼肌细胞胰岛素抵抗的影响。C2C12 成肌细胞分化为肌管 5 天后，在存在 100nM 胰岛素的情况下用淫羊藿苷 (50 和 100µM) 或二甲双胍 (1mM) 处理 24 小时。通过 ELISA 测量培养物中的脂联素产生，通过 Western blot 分析评估 AMP 激活的蛋白激酶 (AMPK)/胰岛素信号通路的激活。淫羊藿苷可显著增加 C2C12 肌管中的脂联素产生。此外，淫羊藿苷明显促进 AMPK 和乙酰辅酶 A 羧化酶 (ACC) 的磷酸化。淫羊藿苷上调了 C2C12 肌管中磷酸肌醇 3-激酶 (PI3K) 的表达和胰岛素受体底物-1 (IRS-1) 的磷酸化。这些结果表明，淫羊藿苷通过调节骨骼肌胰岛素抵抗，具有治疗 T2D 的潜力。
