Chen Jinglou, Xu Jun, Li Jingjing, Du Lifen, Chen Tao, Liu Ping, Peng Sisi, Wang Mingwei, Song Hongping
Department of Pharmacy, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Department of Pharmacy, Puai Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, China.
Int Immunopharmacol. 2015 May;26(1):147-52. doi: 10.1016/j.intimp.2015.03.025. Epub 2015 Mar 31.
Green tea (Camellia sinensis) is an extremely popular beverage worldwide. Epigallocatechin-3-gallate (EGCG) is one of the major catechins isolated from green tea and contributes to its beneficial therapeutic functions including antioxidant, anti-inflammatory and anti-cancer effects. However, the effect of EGCG on mastitis is not yet known. This study was to investigate the protective potential of EGCG against mastitis in rats. The rat mastitis model was induced by injecting lipopolysaccharide (LPS) into the duct of mammary gland. The mammary gland was collected after the experimental period. The levels of mammary oxidative stress and inflammatory responses were assessed by measuring the local activities of antioxidant enzymes and the levels of inflammatory cytokines. The mammary expressions of mitogen-activated protein kinases (MAPKs), nuclear factor κB-p65 (NFκB-p65) and hypoxia-inducible factor-1α (HIF-1α) were evaluated by western blot analysis. It was found that EGCG obviously normalized LPS-induced low activities of antioxidant enzymes as well as decreased the high levels of inflammatory cytokines. Additionally, EGCG inhibited the mammary over-expression of MAPKs, NFκB-p65 and HIF-1α. These results indicated that EGCG was able to attenuate LPS-induced mastitis in rats by suppressing MAPK related oxidative stress and inflammatory responses.
绿茶（茶树）是一种在全球极受欢迎的饮品。表没食子儿茶素没食子酸酯（EGCG）是从绿茶中分离出的主要儿茶素之一，具有抗氧化、抗炎和抗癌等有益治疗功能。然而，EGCG对乳腺炎的影响尚不清楚。本研究旨在探讨EGCG对大鼠乳腺炎的保护作用。通过向乳腺导管注射脂多糖（LPS）建立大鼠乳腺炎模型。实验期结束后采集乳腺组织。通过测量抗氧化酶的局部活性和炎症细胞因子水平来评估乳腺氧化应激和炎症反应水平。通过蛋白质免疫印迹分析评估丝裂原活化蛋白激酶（MAPKs）、核因子κB-p65（NFκB-p65）和缺氧诱导因子-1α（HIF-1α）在乳腺中的表达。结果发现，EGCG能明显使LPS诱导的抗氧化酶低活性恢复正常，并降低炎症细胞因子的高水平。此外，EGCG抑制了MAPKs、NFκB-p65和HIF-1α在乳腺中的过度表达。这些结果表明，EGCG能够通过抑制MAPK相关的氧化应激和炎症反应来减轻LPS诱导的大鼠乳腺炎。
