多铜氧化酶CueO的双突变体Cys500Ser/Glu506Ala中三核铜中心的一种新型静止形式。
A novel resting form of the trinuclear copper center in the double mutant of a multicopper oxidase, CueO, Cys500Ser/Glu506Ala.
作者信息
Kajikawa Takao, Sugiyama Ryosuke, Kataoka Kunishige, Sakurai Takeshi
机构信息
Graduate School of Natural Science and Technology, Kanazawa University, Kakuma, Kanazawa 920-1192, Japan.
Graduate School of Natural Science and Technology, Kanazawa University, Kakuma, Kanazawa 920-1192, Japan.
出版信息
J Inorg Biochem. 2015 Aug;149:88-90. doi: 10.1016/j.jinorgbio.2015.03.005. Epub 2015 Mar 24.
A multicopper oxidase, CueO was doubly mutated at its type I copper ligand, Cys500 and an acidic amino acid residue located in the proton transfer pathway, Glu506, to Ser and Ala, respectively. Cys500Ser/Glu506Ala was mainly in a novel resting form to afford the absorption band at ca. 400 nm and an EPR signal with a highly anisotropic character derived from type III copper. However, Cys500Ser/Glu506Ala gave the same reaction intermediate (peroxide intermediate) as that from Cys500Ser and Cys500Ser/Glu506Gln.
多铜氧化酶CueO在其I型铜配体Cys500以及位于质子转移途径中的酸性氨基酸残基Glu506处分别发生双突变，突变为Ser和Ala。Cys500Ser/Glu506Ala主要处于一种新的静止形式，在约400 nm处产生吸收带以及源自III型铜的具有高度各向异性特征的电子顺磁共振信号。然而，Cys500Ser/Glu506Ala产生的反应中间体（过氧化物中间体）与Cys500Ser和Cys500Ser/Glu506Gln产生的相同。
Zotero 插件