Ito H, Oda N, Ito M, Kameda T, Nakayama H, Tahara E
Hiroshima J Med Sci. 1989 Sep;38(3):121-4.
We examined a human gastric carcinoma cell line TMK-1 cytogenetically by G-banding technique. TMK-1 cells were characterized not only by numerical aberrations but also by structural rearrangements affecting various chromosomes. The modal chromosome number was found to be in the hypotriploid range with most ranging between 64 and 66. Flow cytometry revealed near triploid DNA histogram which was correlated well with chromosome counts. Gain of chromosome 7, 9, 11, and X was found in all of the 25 cells examined and trisomy of chromosome 3, 5, 10, 19, 21 and 22 was also frequently detected. Loss of Y chromosome was shown in all of the cells and monosomy of chromosome 6 and 13 was frequently detected. The most conspicuous rearrangements involved chromosome 6, 12, and 16 which showed complex nonreciprocal translocation. Twenty-two cells (88%) had isochromosome of 12q. A brief review is made of reported karyotype of human gastric cancers.
我们采用G显带技术对人胃癌细胞系TMK-1进行了细胞遗传学研究。TMK-1细胞不仅具有数目畸变，还存在影响各种染色体的结构重排。发现众数染色体数处于亚三倍体范围，多数在64到66之间。流式细胞术显示接近三倍体的DNA直方图，这与染色体计数结果高度相关。在所检测的25个细胞中均发现7号、9号、11号和X染色体增加，3号、5号、10号、19号、21号和22号染色体三体也经常被检测到。所有细胞均显示Y染色体缺失，6号和13号染色体单体也经常被检测到。最明显的重排涉及6号、12号和16号染色体，表现为复杂的非相互易位。22个细胞（88%）有12q等臂染色体。本文对已报道的人胃癌核型进行了简要综述。
