Sato N, Fujii K, Yuge O, Morio M
Hiroshima J Med Sci. 1989 Sep;38(3):131-4.
Pentane was measured in a mixture of guinea pig liver microsomes and carbontetrachloride (CCl4) in the presence of nicotineamide adenine dinucleotide phosphate (NADPH) and under anaerobic conditions by gas chromatography. Pentane of 0.2 pmol/mg protein/min was formed in the presence of NADPH without CCl4. Pentane formation increased to 2.0 pmol/mg protein/min in the presence of CCl4 and NADPH. This reaction required an anaerobic atmosphere, 2.1 mmol/litter of NADPH and 13.4 mmol/litter of CCl4 under optimal conditions. The formation was reduced in the presence of oxygen, glutathione, vitamin E and metyrapone. These results clearly show that pentane is formed by lipid peroxidation initiated by the free radical cleavage products of CCl4, which are anaerobically produced by NADPH-dependent microsomal enzymes. We conclude that pentane is a good index of in vitro lipid peroxidation via microsomal NADPH-P-450 enzyme systems.
在烟酰胺腺嘌呤二核苷酸磷酸（NADPH）存在且厌氧的条件下，通过气相色谱法测定豚鼠肝微粒体与四氯化碳（CCl4）混合物中的戊烷。在无CCl4但有NADPH存在的情况下，戊烷的生成量为0.2皮摩尔/毫克蛋白质/分钟。在CCl4和NADPH存在时，戊烷生成量增加至2.0皮摩尔/毫克蛋白质/分钟。该反应在最佳条件下需要无氧环境、2.1毫摩尔/升的NADPH和13.4毫摩尔/升的CCl4。在有氧、谷胱甘肽、维生素E和甲吡酮存在时，戊烷生成量减少。这些结果清楚地表明，戊烷是由CCl4的自由基裂解产物引发的脂质过氧化反应生成的，这些自由基裂解产物由依赖NADPH的微粒体酶在无氧条件下产生。我们得出结论，戊烷是通过微粒体NADPH - P - 450酶系统进行体外脂质过氧化反应的良好指标。
