Sato N, Fujii K, Kawamoto M, Yuge O, Morio M
Department of Anesthesiology, Hiroshima University School of Medicine, Japan.
Res Commun Chem Pathol Pharmacol. 1990 Oct;70(1):93-101.
Pentane, which is an index of lipid peroxidation, was formed in a mixture of microsomes, carbon tetrachloride (CCl4) and NADPH. Trichloromethyl radical adduct of the N-tert-butyl-alpha-phenylnitrone-trichroromethyl (BPN) was also formed in the mixture with BPN. The formation of pentane and the radical adduct were reduced in the presence of paraquat. Furthermore a spectrophotometric study showed that substrate-induced difference spectra in the Soret region (Type I) caused by CCl4 and microsomes were changed after adding paraquat. We conclude that paraquat inhibits the lipid peroxidation mediated by the radical intermediate of CCl4 by inhibiting the mixed function oxidase system.
