Sweeney C R, Sweeney R W, Benson C E
Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania, Kennett Square 19348.
J Am Vet Med Assoc. 1989 Nov 1;195(9):1225-9.
Results of bacteriologic culture of specimens obtained from 14 horses with pneumonia and from 15 horses that were clinically healthy (nonpneumonia group) were compared. Specimens were obtained by use of an endoscopic guarded tracheal swabbing method and percutaneous tracheal aspiration. The percentage of agreement between the 2 tracheal specimens for the horses of the pneumonia group was 79% for aerobic isolates and 100% for anaerobic isolates. The percentage of agreement between results of the 2 tracheal specimens for horses of the nonpneumonia group was 80% for aerobic organisms and 93% for anaerobic organisms. The endoscopic guarded swabbing method described here for obtaining specimens from the lower airways for bacteriologic culture did not always prevent contamination. Results of the study suggested that this endoscopic swabbing technique has merit and should be considered as an alternative to the tracheal aspiration method. Pseudomonas sp and any anaerobic bacteria that are isolated must be viewed as potential contaminants of the endoscopic swab specimen and may not be involved in the primary pulmonary disease.
对从14匹患肺炎的马以及15匹临床健康的马（非肺炎组）获取的样本进行细菌培养结果比较。样本通过内镜保护气管拭子法和经皮气管抽吸获取。肺炎组马匹的两份气管样本之间，需氧菌分离株的一致性百分比为79%，厌氧菌分离株为100%。非肺炎组马匹的两份气管样本结果之间，需氧菌的一致性百分比为80%，厌氧菌为93%。此处描述的用于从下呼吸道获取样本进行细菌培养的内镜保护拭子法并不总能防止污染。研究结果表明，这种内镜拭子技术有其优点，应被视为气管抽吸法的替代方法。假单胞菌属和任何分离出的厌氧菌必须被视为内镜拭子样本的潜在污染物，可能与原发性肺部疾病无关。
