Lehmann R, Gallert C, Roddelkopf T, Junginger S, Wree A, Thurow K
Center for Life Science Automation (celisca), University of Rostock, Friedrich-Barnewitz Str. 8, 18119, Rostock, Germany.
Institute of Automation, University Rostock, Rostock, Germany.
Cytotechnology. 2016 Aug;68(4):1049-62. doi: 10.1007/s10616-015-9861-1. Epub 2015 Apr 5.
Cancer diseases are a common problem of the population caused by age and increased harmful environmental influences. Herein, new therapeutic strategies and compound screenings are necessary. The regular 2D cultivation has to be replaced by three dimensional cell culturing (3D) for better simulation of in vivo conditions. The 3D cultivation with alginate matrix is an appropriate method for encapsulate cells to form cancer constructs. The automated manufacturing of alginate beads might be an ultimate method for large-scaled manufacturing constructs similar to cancer tissue. The aim of this study was the integration of full automated systems for the production, cultivation and screening of 3D cell cultures. We compared the automated methods with the regular manual processes. Furthermore, we investigated the influence of antibiotics on these 3D cell culture systems. The alginate beads were formed by automated and manual procedures. The automated steps were processes by the Biomek(®) Cell Workstation (celisca, Rostock, Germany). The proliferation and toxicity were manually and automatically evaluated at day 14 and 35 of cultivation. The results visualized an accumulation and expansion of cell aggregates over the period of incubation. However, the proliferation and toxicity were faintly and partly significantly decreased on day 35 compared to day 14. The comparison of the manual and automated methods displayed similar results. We conclude that the manual production process could be replaced by the automation. Using automation, 3D cell cultures can be produced in industrial scale and improve the drug development and screening to treat serious illnesses like cancer.
癌症疾病是由年龄增长和日益严重的有害环境影响导致的常见人群问题。在此，新的治疗策略和化合物筛选是必要的。常规的二维培养必须被三维细胞培养（3D）所取代，以便更好地模拟体内条件。用藻酸盐基质进行三维培养是一种包封细胞以形成癌症构建体的合适方法。藻酸盐珠的自动化制造可能是大规模制造类似于癌组织构建体的最终方法。本研究的目的是集成用于三维细胞培养的生产、培养和筛选的全自动化系统。我们将自动化方法与常规手动操作进行了比较。此外，我们研究了抗生素对这些三维细胞培养系统的影响。藻酸盐珠通过自动化和手动程序形成。自动化步骤由Biomek（®）细胞工作站（德国罗斯托克的celisca公司）进行。在培养的第14天和第35天手动和自动评估细胞增殖和毒性。结果显示在孵育期间细胞聚集体的积累和扩展。然而，与第14天相比，第35天的细胞增殖和毒性略有下降且部分显著降低。手动和自动化方法的比较显示了相似的结果。我们得出结论，手动生产过程可以被自动化所取代。使用自动化，可以大规模生产三维细胞培养物，并改善药物开发和筛选，以治疗像癌症这样的严重疾病。
