基于海藻酸盐的 3D 癌细胞培养用于治疗反应建模。
Alginate-based 3D cancer cell culture for therapeutic response modeling.
机构信息
Massachusetts General Hospital, Center for Cancer Research, Harvard Medical School, 149 13th Street, Boston, MA 02129, USA.
Department of Radiation Oncology, Massachusetts General Hospital, Harvard Medical School, 55 Fruit Street, Boston, MA 02114, USA.
出版信息
STAR Protoc. 2021 Mar 18;2(2):100391. doi: 10.1016/j.xpro.2021.100391. eCollection 2021 Jun 18.
Two-dimensional (2D) culture of tumor cells fails to recapitulate some important aspects of cellular organization seen in experiments. In addition, cell cultures traditionally use non-physiological concentration of nutrients. Here, we describe a protocol for a facile three-dimensional (3D) culture format for cancer cells. This 3D platform helps overcome the 2D culture limitations. In addition, it allows for longitudinal modeling of responses to cancer therapeutics. For complete details on the use and execution of this protocol, please refer to Lhuissier et al. (2017), Lehmann et al. (2016), Liu et al. (2016), and Duval et al. (2011).
二维（2D）培养的肿瘤细胞无法重现实验中观察到的一些重要的细胞组织方面。此外，细胞培养传统上使用非生理浓度的营养物质。在这里，我们描述了一种用于癌症细胞的简便的三维（3D）培养方法。这种 3D 平台有助于克服 2D 培养的局限性。此外，它还可以对癌症治疗药物的反应进行纵向建模。有关此方案的使用和执行的完整详细信息，请参考 Lhuissier 等人（2017 年）、Lehmann 等人（2016 年）、Liu 等人（2016 年）和 Duval 等人（2011 年）。
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