Tierforschungsanlage, Universität Konstanz, 78343, Konstanz, Germany.
Cytotechnology. 1995 Jun;19(2):95-105. doi: 10.1007/BF00749764.
This article describes the historical development of the prophylactic use of antibiotics in cell culture as well as their effects on cells. The influence of antibiotics on cell morphology, cellular degeneration and cell death and cellular function is summarized. Cellular DNA as well as protein synthesis are affected which can lead to interference with, or even changes in, metabolic processes. Such effects must be considered in cell culture research. As antibiotics are used in multifold ways, the otherwise standardized conditions in cell culture are no longer comparable. The prophylactic use of antibiotics is rejected for scientific reasons.
本文描述了抗生素在细胞培养中的预防性使用及其对细胞的影响的历史发展。总结了抗生素对细胞形态、细胞变性和细胞死亡以及细胞功能的影响。细胞 DNA 以及蛋白质合成受到影响，这可能导致代谢过程受到干扰，甚至发生变化。在细胞培养研究中必须考虑到这些影响。由于抗生素的使用方式多种多样，否则细胞培养中的标准化条件不再具有可比性。出于科学原因，拒绝预防性使用抗生素。
