Yousif Nasser Ghaly, Sadiq Alaa Muhammad, Yousif Maitham G, Al-Mudhafar Rihab H, Al-Baghdadi Jinan J, Hadi Najah
Department of Medicine and Surgery, University of Colorado Denver, Denver, CO, 80045, USA,
Arch Gynecol Obstet. 2015 Oct;292(4):899-904. doi: 10.1007/s00404-015-3694-1. Epub 2015 Apr 5.
Notch signalings are regulated multiple cellular processes during cancer progression. We aimed to investigate the significance and prognostic value of expression of Notch1 and JAG1 in cervical cancer to determine whether they could serve as prognostic predictors.
METHODS/MATERIALS: The expression of Notch1/JAGD1 was investigated by real-time PCR, western blot assay and its association with overall survival of patients was analyzed by statistical analysis.
Notch1 and JAGD1 expression level were significantly elevated in cervical cancer in comparison to normal specimens and other types of Notch receptors and ligands. It is also proved that Notch1 and JAGD1 expression were to be associated with cervical cancer invasion, lymph node metastasis, and FIGO system. In addition, survival analysis proved that elevated Notch1 and JAGD1 expression were associated with poor overall survival of patients (P = 0.01, P = 0.02 log-rank test), respectively.
The present data proved the over-expression of Notch1/JAGD1 and its association with tumor progression in human cervical cancer, which might be a potential valuable biomarker for cervical cancer and further studies need.
Notch信号通路在癌症进展过程中受到多种细胞过程的调控。我们旨在研究Notch1和JAG1在宫颈癌中的表达意义及预后价值，以确定它们是否可作为预后预测指标。
方法/材料：采用实时PCR、蛋白质免疫印迹法检测Notch1/JAGD1的表达，并通过统计学分析其与患者总生存期的关系。
与正常标本及其他类型的Notch受体和配体相比，宫颈癌中Notch1和JAGD1的表达水平显著升高。还证实Notch1和JAGD1的表达与宫颈癌侵袭、淋巴结转移及国际妇产科联盟（FIGO）分期有关。此外，生存分析表明，Notch1和JAGD1表达升高分别与患者总生存期较差相关（P = 0.01，P = 0.02，对数秩检验）。
目前的数据证实了Notch1/JAGD1在人类宫颈癌中的过度表达及其与肿瘤进展的关系，这可能是宫颈癌潜在的有价值生物标志物，有待进一步研究。
