Vorob'eva L I, Rogozhin E A, Khodzhaev E Iu, Nikolaev I V, Turova T P
Prikl Biokhim Mikrobiol. 2015 Jan-Feb;51(1):37-45. doi: 10.7868/s0555109914060166.
It has been shown that a producer strain of reactivating factor (RF) is identical to a typical strain of Luteococcus japonicus DSM 10546 from the Propionibacteriaceae family according to the physiological and biochemical properties and the sequencing of 16S rRNA fragments. A number of phenotypical differences from the model strain allowed the producer strain to be considered a subspecies of Luteococcus japonicus, and it was named Luteococcus japonicus subsp. casei. At cultivation of the producer, RF is secreted into the medium and plays the role of a signaling molecule. RF antioxidant activities towards various organic radicals may be a possible mechanism of its protective and reactivating effects. Metabolites secreted by the L. casei producer strain into the culture medium were separated by a combination of liquid chromatographies. Four components possessing biological activities were found. The most active one was studied by MALDI-TOF mass spectrometry, which revealed that it is a polypeptide. Primary identification of some amino acid residues was performed. Sugar residues were found in the structure.
研究表明，根据生理生化特性以及16S rRNA片段测序结果，一种再激活因子（RF）产生菌与丙酸杆菌科的日本黄体球菌DSM 10546典型菌株相同。该产生菌与模式菌株存在一些表型差异，因此被视为日本黄体球菌的一个亚种，并命名为日本黄体球菌酪亚种。在培养该产生菌时，RF分泌到培养基中并发挥信号分子的作用。RF对各种有机自由基的抗氧化活性可能是其具有保护和再激活作用的一种机制。通过液相色谱联用技术分离了酪亚种产生菌分泌到培养基中的代谢产物。发现了四种具有生物活性的成分。对活性最高的成分进行了基质辅助激光解吸电离飞行时间质谱分析，结果表明它是一种多肽。对一些氨基酸残基进行了初步鉴定。在其结构中发现了糖残基。
