Marcus D M, Latov N, Hsi B P, Gillard B K
Department of Medicine, Baylor College of Medicine, Houston, TX 77030.
J Neuroimmunol. 1989 Dec;25(2-3):255-9. doi: 10.1016/0165-5728(89)90144-6.
Twelve laboratories from the United States, Canada, France, Italy and Switzerland participated in a workshop to compare assays used to measure anti-GM1 antibodies, and to discuss the clinical significance of these antibodies. A panel of test samples containing varying amounts of anti-GM1 antibody was prepared by mixing varied proportions of normal serum with a serum containing a monoclonal IgM antibody that bound GM1 ganglioside. Enzyme-linked immunosorbent assay (ELISA) data were supplied by eight laboratories and ten laboratories classified the sera as negative, weakly or strongly positive. Most laboratories correctly identified the two samples that contained the highest quantities of antibody, but there was considerable disagreement on the classification of the three samples with moderate or small amounts of antibody. The sensitivity of the assays varied considerably. The more sensitive assays did not use detergent in the washing buffers, and incubated the human serum with the antigen at 4 degrees C overnight. Several investigators have identified a subset of patients with lower motor neuron disease or multifocal neuropathy who have high titers of anti-GM1 antibodies. Many patients with neurological and non-neurological diseases have low to moderate levels of anti-GM1 antibodies, and the significance of these antibodies is unclear. There was general agreement that standardization of the ELISA assays is urgently required, and that distribution of a reference high-titered antiserum would facilitate this process.
来自美国、加拿大、法国、意大利和瑞士的12个实验室参与了一场研讨会，以比较用于检测抗GM1抗体的检测方法，并讨论这些抗体的临床意义。通过将不同比例的正常血清与含有结合GM1神经节苷脂的单克隆IgM抗体的血清混合，制备了一组含有不同量抗GM1抗体的测试样品。八个实验室提供了酶联免疫吸附测定（ELISA）数据，十个实验室将血清分类为阴性、弱阳性或强阳性。大多数实验室正确识别出了两个抗体含量最高的样品，但对于三个抗体含量中等或较少的样品的分类存在很大分歧。这些检测方法的灵敏度差异很大。更灵敏的检测方法在洗涤缓冲液中不使用去污剂，并在4摄氏度下将人血清与抗原孵育过夜。几位研究人员已经确定了一部分患有下运动神经元疾病或多灶性神经病的患者，他们的抗GM1抗体滴度很高。许多患有神经和非神经疾病的患者抗GM1抗体水平低至中等，这些抗体的意义尚不清楚。大家普遍认为，ELISA检测方法的标准化迫切需要，分发一份高滴度参考抗血清将有助于这一过程。
