Sutherland C G, al-Badri A M, Howatson A G, Farquharson M A, Foulis A K
Department of Pathology, Royal Infirmary, Glasgow, Scotland.
J Clin Pathol. 1989 Oct;42(10):1065-9. doi: 10.1136/jcp.42.10.1065.
The expression of immunoreactive alpha interferon was examined in 78 liver biopsy specimens using an indirect immunoperoxidase technique. Biopsy specimens included cases of acute viral hepatitis, chronic active hepatitis, primary biliary cirrhosis, alcoholic hepatitis, large bile duct obstruction and normal liver. Kupffer cells were positive for alpha interferon in all cases. Hepatocytes were negative for alpha interferon in normal liver but in acute viral hepatitis were positive in perivenular and necrotic areas. Hepatocytes were positive in periportal areas, associated with piecemeal necrosis, in chronic active hepatitis and primary biliary cirrhosis, and were positive in perivenular areas in alcoholic hepatitis and large bile duct obstruction. The unexpected finding of alpha interferon in hepatocytes in non-viral liver disease indicates that the presence of this substance in liver cells cannot be taken as a specific marker of viral infection.
采用间接免疫过氧化物酶技术，对78份肝活检标本中免疫反应性α干扰素的表达情况进行了检测。活检标本包括急性病毒性肝炎、慢性活动性肝炎、原发性胆汁性肝硬化、酒精性肝炎、大胆管梗阻及正常肝脏病例。在所有病例中，枯否细胞α干扰素呈阳性。正常肝脏中的肝细胞α干扰素呈阴性，但在急性病毒性肝炎中，肝小叶中央静脉周围及坏死区域的肝细胞呈阳性。在慢性活动性肝炎和原发性胆汁性肝硬化中，汇管区周围伴有桥接坏死的肝细胞呈阳性，在酒精性肝炎和大胆管梗阻中，肝小叶中央静脉周围的肝细胞呈阳性。在非病毒性肝病的肝细胞中意外发现α干扰素，这表明肝细胞中该物质的存在不能被视为病毒感染的特异性标志物。
