Li Ai-Ping, Li Zhen-Yu, Sun Hai-Feng, Li Ke, Qin Xue-Mei, Du Guan-Hua
§Institute of Materia Medica, Chinese Academy of Medical Sciences, Beijing 100050, People's Republic of China.
J Proteome Res. 2015 May 1;14(5):2005-16. doi: 10.1021/pr501167u. Epub 2015 Apr 16.
Astragali Radix (AR) is a commonly used herbal drug in traditional chinese medicine and is widely used for the treatment of diabetes, cardiovascular diseases, nephropathy, and neuropathy. The main source of AR in China is the dried root of Astragalus membranaceus var. mongholicus (Bge.) Hsiao, and both cultivated and wild ARs are used clinically. A systematic comparison of cultivated AR (GS-AR) and wild AR (SX-AR) should be performed to ensure the clinical efficacy and safety. In this study, the chemical composition of the two different ARs, which were collected in the Shanxi (wild) and Gansu (cultivated) provinces, were compared by NMR-based metabolic fingerprint coupled with multivariate analysis. The SX-AR- and GS-AR-induced metabolic changes in the endogenous metabolites in mice were also compared. The results showed that SX-AR and GS-AR differed significantly not only in the primary metabolites but also in the secondary metabolites. However, alterations among the endogenous metabolites in the serum, lung, liver, and spleen were relatively small. This study provided a novel and valuable method for the evaluation of the consistency and diversity of herbal drugs, and further studies should be conducted on the difference in polysaccharides as well as the biological effects between the two kinds of AR.
黄芪是中药中常用的草药，广泛用于治疗糖尿病、心血管疾病、肾病和神经病变。中国黄芪的主要来源是蒙古黄芪干燥根，栽培黄芪和野生黄芪均用于临床。应对栽培黄芪（GS-AR）和野生黄芪（SX-AR）进行系统比较，以确保临床疗效和安全性。本研究采用基于核磁共振的代谢指纹图谱结合多变量分析，比较了采自山西（野生）和甘肃（栽培）两省的两种不同黄芪的化学成分。还比较了SX-AR和GS-AR对小鼠体内内源性代谢物的代谢变化影响。结果表明，SX-AR和GS-AR不仅在初级代谢产物上有显著差异，在次级代谢产物上也有显著差异。然而，血清、肺、肝和脾中内源性代谢物的变化相对较小。本研究为评估草药的一致性和多样性提供了一种新颖且有价值的方法，后续还应对两种黄芪的多糖差异及其生物学效应展开进一步研究。
