Day John C, Broughton Richard K, Hinsley Shelley A
a Centre for Ecology and Hydrology (CEH) Wallingford , Wallingford , Oxfordshire , UK.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Sep;27(5):3212-3. doi: 10.3109/19401736.2015.1007351. Epub 2015 Apr 7.
The complete mitochondrial genome of the marsh tit Poecile palustris (Linnaeus, 1758) was sequenced using a combined Illumina and Sanger sequencing approach. Using the known sequence of Poecile atricapillus Linnaeus, 1766 (Paridae) homologous NGS reads were identified and assembled. The genome is 16,824 bp in length and includes 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and a control region. Gene order resembles that of the standard avian gene order. The base composition of the genome is A (29.15%), T (22.50%), C (33.61%) and G (14.73%). The control region between tRNA(Glu) and tRNA(Phe) is composed of 1240 bp with no obvious repetitive motifs.
采用Illumina和桑格测序相结合的方法，对沼泽山雀（Poecile palustris，林奈，1758）的完整线粒体基因组进行了测序。利用已知的褐头山雀（Poecile atricapillus，林奈，1766，山雀科）序列，鉴定并组装了同源的二代测序（NGS） reads。该基因组长度为16,824 bp，包括13个蛋白质编码基因、2个核糖体RNA基因、22个转运RNA基因和一个控制区。基因排列顺序与标准鸟类基因顺序相似。基因组的碱基组成为A（29.15%）、T（22.50%）、C（33.61%）和G（14.73%）。tRNA（Glu）和tRNA（Phe）之间的控制区由1240 bp组成，没有明显的重复基序。
