Prosdocimi Francisco, Souto Helena Magarinos, Ruschi Piero Angeli, Furtado Carolina, Jennings W Bryan
a Laboratório de Genômica e Biodiversidade , Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro , Rio de Janeiro , Brazil .
b Setor de Ornitologia, Departamento de Vertebrados , Museu Nacional, Universidade Federal do Rio de Janeiro , Rio de Janeiro , Brazil .
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Sep;27(5):3214-5. doi: 10.3109/19401736.2015.1007352. Epub 2015 Mar 11.
The genome of the versicoloured emerald hummingbird (Amazilia versicolor) was partially sequenced in one-sixth of an Illumina HiSeq lane. The mitochondrial genome was assembled using MIRA and MITObim software, yielding a circular molecule of 16,861 bp in length and deposited in GenBank under the accession number KF624601. The mitogenome contained 13 protein-coding genes, 22 transfer tRNAs, 2 ribosomal RNAs and 1 non-coding control region. The molecule was assembled using 21,927 sequencing reads of 100 bp each, resulting in ∼130 × coverage of uniformly distributed reads along the genome. This is the forth mitochondrial genome described for this highly diverse family of birds and may benefit further phylogenetic, phylogeographic, population genetic and species delimitation studies of hummingbirds.
在Illumina HiSeq测序通道的六分之一通量下对杂色翠蜂鸟（Amazilia versicolor）的基因组进行了部分测序。使用MIRA和MITObim软件组装线粒体基因组，得到一个长度为16,861 bp的环状分子，并以登录号KF624601存入GenBank。该线粒体基因组包含13个蛋白质编码基因、22个转运tRNA、2个核糖体RNA和1个非编码控制区。该分子使用21,927条长度为100 bp的测序读段进行组装，从而沿着基因组产生了约130倍覆盖度的均匀分布读段。这是针对这个高度多样化鸟类家族所描述的第四个线粒体基因组，可能会有助于蜂鸟进一步的系统发育、系统地理学、群体遗传学和物种界定研究。
