Wei Bin, Shi Haitao, Lu Xiaolan, Shi Ameng, Cheng Yan, Dong Lei
Department of Gastroenterology, Xi'an No. 1 Hospital, Xi'an, Shaanxi 710002, P.R. China.
Department of Gastroenterology, The Second Affiliated Hospital of Medical School, Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China.
Mol Med Rep. 2015 Aug;12(2):2075-81. doi: 10.3892/mmr.2015.3592. Epub 2015 Apr 2.
T-cadherin has been identified as a tumor-suppressor gene in several types of cancer. The present study aimed to investigate the association of the expression of T-cadherin with angiogenesis, and to evaluate its prognostic value for patients with primary gastric cancer. Gastric cancer tissues and matched adjacent tissues from 166 patients receiving surgical resection were included in the present study. The expression of T-cadherin was detected using immunohistochemistry, western blotting and reverse transcription-quantitative polymerase chain reaction. The expression of vascular epidermal growth factor (VEGF) was detected using immunohistochemistry, and its association with the expression of T-cadherin was analyzed. In addition, the association between the expression of T-cadherin and clinicopathological features were analyzed. The mRNA and protein expression levels of T-cadherin were significantly lower in the gastric cancer tissue compared with the corresponding adjacent normal tissue (P<0.05). The expression of VEGF was not associated with the expression of T-cadherin in the gastric cancer tissue. The decreased protein expression of T-cadherin correlated with smoking, larger tumor size (diameter, >4 cm), lymph node metastasis and a higher tumor-lymph node-metastasis stage (P<0.05 or P<0.01). However, the expression of T-cadherin was not correlated with gender, age, alcohol intake, Helecobacter pylori infection or differentiation (P>0.05). The multivariate analysis demonstrated that the expression of T-cadherin was an independent prognostic factor for the overall survival rate of patients with gastric cancer. This data suggested that the downregulation of T-cadherin may contribute to gastric cancer progression, representing a useful biomarker for predicting the biological behavior and prognosis of gastric cancer. However, no significant association was observed between the expression of VEGF and T-cadherin.
T-钙黏蛋白已被确定为多种癌症中的一种肿瘤抑制基因。本研究旨在探讨T-钙黏蛋白表达与血管生成之间的关联，并评估其对原发性胃癌患者的预后价值。本研究纳入了166例接受手术切除的患者的胃癌组织及配对的癌旁组织。采用免疫组织化学、蛋白质印迹法和逆转录-定量聚合酶链反应检测T-钙黏蛋白的表达。采用免疫组织化学检测血管内皮生长因子（VEGF）的表达，并分析其与T-钙黏蛋白表达的关联。此外，分析了T-钙黏蛋白表达与临床病理特征之间的关联。与相应的癌旁正常组织相比，胃癌组织中T-钙黏蛋白的mRNA和蛋白表达水平显著降低（P<0.05）。胃癌组织中VEGF的表达与T-钙黏蛋白的表达无关。T-钙黏蛋白蛋白表达降低与吸烟、肿瘤较大（直径>4 cm）、淋巴结转移及较高的肿瘤-淋巴结-转移分期相关（P<0.05或P<0.01）。然而，T-钙黏蛋白的表达与性别、年龄、饮酒、幽门螺杆菌感染或分化无关（P>0.05）。多因素分析表明，T-钙黏蛋白的表达是胃癌患者总生存率的独立预后因素。该数据表明，T-钙黏蛋白的下调可能促进胃癌进展，是预测胃癌生物学行为和预后的有用生物标志物。然而，未观察到VEGF表达与T-钙黏蛋白之间存在显著关联。
