Peng Qiu-xian, Cai Hong-bing, Peng Jiang-li, Yung Kin Lam, Shi Jue, Mo Zhi-xian
School of Chinese Medicine, Southern Medical University, Guangzhou, 510515, China.
Faculty of Science, Hong Kong Baptist University, Hong Kong SAR, China.
Chin J Integr Med. 2015 Nov;21(11):837-45. doi: 10.1007/s11655-015-2043-3. Epub 2015 Apr 6.
To observe the effects of water extract of Zuojin Pill ([characters: see text], ZJP) on inhibiting the growth of human gastric cancer cell line SGC-7901 and its potential mechanism.
Effects of ZJP on SGC-7901 cells growth were determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay, cell apoptosis and cell cycle were determined by flow cytometry, and apoptosis induction was detected by means of DNA gel electrophoresis. The cellular mechanism of drug-induced cell death was unraveled by assaying oxidative injury level of SGC-7901 cell, mitochondrial membrane potentials, expression of apoptosis-related genes, such as B cell lymphoma/lewkmia-2 (Bcl-2), Bcl-2 associated X protein (Bax) and cleaved caspase-3 and caspase-9.
ZJP exerted evident inhibitory effect on SGC-7901 cells by activating production of reactive oxygen species and elevating Bax/Bcl-2 ratio in SGC-7901 cells, leading to attenuation of mitochondrial membrane potential and DNA fragmentation.
ZJP inhibits the cancer cell growth via activating mitochondria-dependent apoptosis pathway. ZJP can potentially serve as an antitumor agent.
观察左金丸水提取物对人胃癌细胞系SGC - 7901生长的抑制作用及其潜在机制。
采用3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2-H-四氮唑溴盐（MTT）法检测左金丸对SGC - 7901细胞生长的影响，通过流式细胞术检测细胞凋亡和细胞周期，采用DNA凝胶电泳检测凋亡诱导情况。通过检测SGC - 7901细胞的氧化损伤水平、线粒体膜电位以及凋亡相关基因如B细胞淋巴瘤/白血病-2（Bcl-2）、Bcl-2相关X蛋白（Bax）和裂解的半胱天冬酶-3和半胱天冬酶-9的表达，揭示药物诱导细胞死亡的细胞机制。
左金丸通过激活活性氧的产生并提高SGC - 7901细胞中Bax/Bcl-2比值，对SGC - 7901细胞发挥明显的抑制作用，导致线粒体膜电位衰减和DNA片段化。
左金丸通过激活线粒体依赖性凋亡途径抑制癌细胞生长。左金丸有可能作为一种抗肿瘤药物。
