Lin Jie, Wang Guoquan, Li Cuijuan, Sun Lijun
Department of Prescriptions, Basic Medicine School, Shaanxi University of Chinese Medicine, Xianyang 712046, China. *Corresponding author, E-mail:
Department of Prescriptions, Basic Medicine School, Shaanxi University of Chinese Medicine, Xianyang 712046, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2020 Oct;36(10):911-917.
Objective To observe the effect of serum of SD rats lavaged by modified Zuojin decoction on the apoptosis and proliferation of human gastric cancer cells and its mechanism. Methods SD rats were gavaged with modified Zuojin decoction to prepare their sera. Human SGC-7901 and MKN-45 cells were cultured and treated with the sera (0, 25, 50, 100, 200, 400) mL/L. MTT assay was used to observe the effect of drug-containing serum on the proliferation of human gastric cancer cells. Immunofluorescence method was used to detect the expression of ki67 after treatment with the drug-containing serum. The effect of drug-containing serum on the apoptosis of gastric cancer cells was detected by flow cytometry. Western blot analysis was used to detect the protein levels of apoptosis-associated cleaved caspase-3, cleaved caspase-8, cleaved caspase-9, BAX and Bcl2 in SGC-7901 and MKN-45 cells. Results The drug-containing serum significantly inhibited the proliferation and induced the apoptosis of SGC-7901 and MKN-45 cells, and the positive rate of ki67 expression was significantly reduced. The levels of cleaved caspase-3, cleaved caspase-9 and BAX proteins in SGC-7901 and MKN-45 cells increased and the levels of Bcl2 protein decreased. Conclusion The drug-containing serum can significantly inhibit the proliferation and induce the apoptosis of human gastric cancer SGC-7901 and MKN-45 cells, and the mechanism may be related to the activation of mitochondrial pathways.
目的 观察经改良左金汤灌胃的SD大鼠血清对人胃癌细胞凋亡及增殖的影响及其机制。方法 用改良左金汤灌胃SD大鼠以制备血清。培养人SGC-7901和MKN-45细胞，并用血清（0、25、50、100、200、400）mL/L进行处理。采用MTT法观察含药血清对人胃癌细胞增殖的影响。采用免疫荧光法检测含药血清处理后ki67的表达。通过流式细胞术检测含药血清对胃癌细胞凋亡的影响。采用蛋白质免疫印迹分析检测SGC-7901和MKN-45细胞中凋亡相关的裂解型半胱天冬酶-3、裂解型半胱天冬酶-8、裂解型半胱天冬酶-9、BAX和Bcl2的蛋白水平。结果 含药血清显著抑制SGC-7901和MKN-45细胞的增殖并诱导其凋亡，且ki67表达阳性率显著降低。SGC-7901和MKN-45细胞中裂解型半胱天冬酶-3、裂解型半胱天冬酶-9和BAX蛋白水平升高，Bcl2蛋白水平降低。结论 含药血清可显著抑制人胃癌SGC-7901和MKN-45细胞的增殖并诱导其凋亡，其机制可能与线粒体途径的激活有关。
