Saad Ahmed S
Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr-Elaini Street, 11562 Cairo, Egypt.
Spectrochim Acta A Mol Biomol Spectrosc. 2015 Aug 5;147:257-61. doi: 10.1016/j.saa.2015.03.095. Epub 2015 Mar 31.
A simple selective spectrophotometric method for determination of compounds in ternary mixture was developed by combining the resolution power of two well-known methods that are commonly used for binary mixtures; namely ratio difference method and dual wavelength. The new method (dual wavelength in ratio spectra) was successfully applied for the determination of a ternary mixture of betamethasone dipropionate (BM), clotrimazole (CT) and benzyl alcohol (BA) in pure powder form and in their pharmaceutical preparation. The difference in amplitudes (ΔP) in the ratio spectra at 252.0 and 258.0 nm (ΔP(252.0-258.0 nm)) corresponds to BM, while ΔP(266.8-255.4 nm) and ΔP(254.2-243.5 nm) corresponds to CT and BA, respectively. The method was validated as per the USP 2005 guidelines. The developed method can be used in quality control laboratories for routine analysis of compounds in ternary mixtures.
通过结合两种常用于二元混合物的著名方法（即比率差法和双波长法）的分辨能力，开发了一种用于测定三元混合物中化合物的简单选择性分光光度法。新方法（比率光谱中的双波长法）成功应用于测定纯粉末形式及其药物制剂中的倍他米松二丙酸酯（BM）、克霉唑（CT）和苯甲醇（BA）的三元混合物。252.0和258.0 nm处比率光谱的振幅差（ΔP）（ΔP(252.0 - 258.0 nm)）对应于BM，而ΔP(266.8 - 255.4 nm)和ΔP(254.2 - 243.5 nm)分别对应于CT和BA。该方法按照美国药典2005版指南进行了验证。所开发的方法可用于质量控制实验室对三元混合物中化合物的常规分析。
