Yuan Liu, Wu Yu, Gu Qi-Sheng, El-Hamshary Hany, El-Newehy Mohamed, Mo Xiumei
State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Materials Science and Engineering, Donghua University, Shanghai 201620, China; Biomaterials and Tissue Engineering Lab, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
Biomaterials and Tissue Engineering Lab, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
Int J Biol Macromol. 2017 Mar;96:569-577. doi: 10.1016/j.ijbiomac.2016.12.058. Epub 2016 Dec 23.
Recently, photocrosslinked hydrogels have attracted more and more attention in biomedical applications. In this study, a serials of injectable hydrogels were fabricated from aldehyde methacrylate sodium alginate and amino gelatin (AMSA/AG) using a two-step process. Here, sodium alginate, a kind of natural polysaccharide, was modified by oxidizer to form aldehyde sodium alginate (ASA), and methacrylate groups were further grafted on the main chain of ASA. Gelatin, the denatured form of collagen, was modified with ethylenediamine (ED) to graft more amino groups. When AMSA and AG aqueous solutions were mixed, the Schiff base reaction occurred quickly to form the primary network between aldehyde groups in AMSA and amino groups in AG, and then a 365nm ultraviolet (UV) light was used to initiate the radical reaction of methacrylate groups in AMSA to produce the secondary network. The structures and properties of AMSA/AG hydrogels were evaluated by Fourier Transforms Infrared spectroscopy (FTIR) and HNMR analysis. The swelling ratio confirmed the density of crosslinked networks, and the mechanical performance demonstrated that the UV initiated the double crosslinking network hydrogels have an improved mechanical properties compared to the single Schiff base networks hydrogels. The results showed that the photocrosslinked double network hydrogels have enhanced mechanical properties, good biocompatibility and controllable degradation rate. So, this hydrogels may have great potential utilized in regenerative medicine as therapeutic materials.
近年来，光交联水凝胶在生物医学应用中越来越受到关注。在本研究中，采用两步法由甲基丙烯酸醛基海藻酸钠和氨基明胶（AMSA/AG）制备了一系列可注射水凝胶。在此，海藻酸钠作为一种天然多糖，经氧化剂改性形成醛基海藻酸钠（ASA），并在ASA主链上进一步接枝甲基丙烯酸酯基团。明胶是胶原蛋白的变性形式，用乙二胺（ED）进行改性以接枝更多氨基。当AMSA和AG水溶液混合时，席夫碱反应迅速发生，在AMSA中的醛基和AG中的氨基之间形成初级网络，然后用365nm紫外光引发AMSA中甲基丙烯酸酯基团的自由基反应以产生次级网络。通过傅里叶变换红外光谱（FTIR）和核磁共振氢谱（HNMR）分析对AMSA/AG水凝胶的结构和性能进行了评估。溶胀率证实了交联网络的密度，力学性能表明，与单席夫碱网络水凝胶相比，紫外光引发的双交联网络水凝胶具有改善的力学性能。结果表明，光交联双网络水凝胶具有增强的力学性能、良好的生物相容性和可控的降解速率。因此，这种水凝胶作为治疗材料在再生医学中可能具有巨大的应用潜力。
