Liu Hongjun, Yang Xuerong, Liao Xinhui, Zuo Tao, Qin Cheng, Cao Shiliang, Dong Ling, Zhou Huangkai, Zhang Yongzhong, Liu Sisi, Shen Yaou, Lin Haijian, Lübberstedt Thomas, Zhang Zhiming, Pan Guangtang
Maize Research Institute of Sichuan Agricultural University, Chengdu 611130, China.
Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, China.
Genomics. 2015 Jul;106(1):52-60. doi: 10.1016/j.ygeno.2015.03.005. Epub 2015 Apr 3.
The present study profiled and analyzed gene expression of the maize ear at four key developmental stages. Based on genome-wide profile analysis, we detected differential mRNA of maize genes. Some of the differentially expressed genes (DEGs) were predicted to be potential candidates of maize ear development. Several well-known genes were found with reported mutant analyses, such as, compact plant2 (ct2), zea AGAMOUS homolog1 (zag1), bearded ear (bde), and silky1 (si1). MicroRNAs such as microRNA156 were predicted to target genes involved in maize ear development. Antisense transcripts were widespread throughout all the four stages, and are suspected to play important roles in maize ear development. Thus, identification and characterization of important genes and regulators at all the four developmental stages will contribute to an improved understanding of the molecular mechanisms responsible for maize ear development.
本研究对玉米果穗在四个关键发育阶段的基因表达进行了分析和描述。基于全基因组分析，我们检测到了玉米基因的差异mRNA。一些差异表达基因（DEGs）被预测为玉米果穗发育的潜在候选基因。通过已报道的突变分析发现了几个知名基因，如紧凑型植株2（ct2）、玉米AGAMOUS同源基因1（zag1）、有髯果穗（bde）和丝穗1（si1）。预测诸如microRNA156等微小RNA靶向参与玉米果穗发育的基因。反义转录本在所有四个阶段均广泛存在，并被怀疑在玉米果穗发育中发挥重要作用。因此，鉴定和表征这四个发育阶段的重要基因和调控因子将有助于更好地理解玉米果穗发育的分子机制。
