Wang Zhongying, Tonderys Daniel, Leggett Susan E, Williams Evelyn Kendall, Kiani Mehrdad T, Steinberg Ruben Spitz, Qiu Yang, Wong Ian Y, Hurt Robert H
School of Engineering, Brown University, Providence, RI 02912 ; Department of Chemistry, Brown University, Providence, RI 02912.
School of Engineering, Brown University, Providence, RI 02912 ; Center for Biomedical Engineering, Brown University, Providence, RI 02912.
Carbon N Y. 2016 Feb 1;97:14-24. doi: 10.1016/j.carbon.2015.03.040.
Textured surfaces with periodic topographical features and long-range order are highly attractive for directing cell-material interactions. They mimic physiological environments more accurately than planar surfaces and can fundamentally alter cell alignment, shape, gene expression, and cellular assembly into superstructures or microtissues. Here we demonstrate for the first time that wrinkled graphene-based surfaces are suitable as textured cell attachment substrates, and that engineered wrinkling can dramatically alter cell alignment and morphology. The wrinkled surfaces are fabricated by graphene oxide wet deposition onto pre-stretched elastomers followed by relaxation and mild thermal treatment to stabilize the films in cell culture medium. Multilayer graphene oxide films form periodic, delaminated buckle textures whose wavelengths and amplitudes can be systematically tuned by variation in the wet deposition process. Human and murine fibroblasts attach to these textured films and remain viable, while developing pronounced alignment and elongation relative to those on planar graphene controls. Compared to lithographic patterning of nanogratings, this method has advantages in the simplicity and scalability of fabrication, as well as the opportunity to couple the use of topographic cues with the unique conductive, adsorptive, or barrier properties of graphene materials for functional biomedical devices.
具有周期性地形特征和长程有序的纹理表面对于引导细胞与材料的相互作用极具吸引力。它们比平面表面更准确地模拟生理环境，并且可以从根本上改变细胞排列、形状、基因表达以及细胞组装成超结构或微组织。在此，我们首次证明基于石墨烯的褶皱表面适合作为有纹理的细胞附着底物，并且工程化的褶皱可以显著改变细胞排列和形态。通过将氧化石墨烯湿沉积到预拉伸的弹性体上，随后进行松弛和温和热处理以使薄膜在细胞培养基中稳定，从而制备出褶皱表面。多层氧化石墨烯薄膜形成周期性的、分层的扣状纹理，其波长和幅度可以通过湿沉积过程中的变化进行系统调节。人类和小鼠成纤维细胞附着在这些有纹理的薄膜上并保持活力，同时相对于平面石墨烯对照，它们会形成明显的排列和伸长。与纳米光栅的光刻图案化相比，该方法在制造的简单性和可扩展性方面具有优势，并且有机会将地形线索的使用与石墨烯材料独特的导电、吸附或阻隔特性相结合，用于功能性生物医学设备。
