伊朗沙赫雷克德临床标本中分离出的肺炎克雷伯菌magA基因的流行率评估及药敏性分析
Prevalence assessment of magA gene and antimicrobial susceptibility of Klebsiella pneumoniae isolated from clinical specimens in Shahrekord, Iran.
作者信息
Amraie Hadis, Shakib Pegah, Rouhi Samaneh, Bakhshandeh Neda, Zamanzad Behnam
机构信息
Department of Microbiology, Lorestan University of Medical Sciences, Khorramabad, Iran.
Cellular & Molecular Research Center and Microbiology Department, Member of Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran.
出版信息
Iran J Microbiol. 2014 Oct;6(5):311-6.
BACKGROUND AND OBJECTIVES
Klebsiella pneumoniae (K. pneumoniae) is an opportunistic microorganism. This study aimed to investigate the presence of magA gene and antimicrobial susceptibility in K. pneumoniae.
MATERIALS AND METHODS
clinical specimens were collected from hospitals of Shahrekord, Iran. Bacterial culture, biochemical diagnostic standard test, determination of antibiotic sensitivity, phenotypic testing hypermucoviscosity (HV) and polymerase chain reaction (PCR) was performed for isolation and characterization of K. pneumoniae.
RESULTS
173 samples were positive for K. pneumoniae. The highest and lowest rates of resistance were related to amoxicillin 79.19% and ciprofloxacin 15.60%, respectively. Also 4 samples were positive for magA gene.
CONCLUSION
Based on our results, K. pneumoniae strains were resistant to different antibiotics. Knowing how to identify strains of K. pneumoniae, spreading of its virulence and also antimicrobial resistance genes can be useful in treatment of infection caused by this bacterium.
背景与目的
肺炎克雷伯菌是一种机会致病菌。本研究旨在调查肺炎克雷伯菌中magA基因的存在情况及抗菌药敏性。
材料与方法
从伊朗沙赫雷克德的医院收集临床标本。进行细菌培养、生化诊断标准试验、抗生素敏感性测定、高黏液性（HV）表型检测及聚合酶链反应（PCR），以分离和鉴定肺炎克雷伯菌。
结果
173份样本肺炎克雷伯菌呈阳性。耐药率最高和最低的分别是阿莫西林79.19%和环丙沙星15.60%。另外，4份样本magA基因呈阳性。
结论
根据我们的结果，肺炎克雷伯菌菌株对不同抗生素耐药。了解如何鉴定肺炎克雷伯菌菌株、其毒力传播情况以及抗菌耐药基因，可能有助于治疗由该细菌引起的感染。
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