Shakib Pegah, Ramazanzadeh Rashid, Taherikalani Morovat, Nouri Bijan
Cellular and Molecular Research Center & Department of Microbiology, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Razi Herbal Medicines Research Center & Department of Microbiology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran.
Infect Disord Drug Targets. 2018;18(2):156-163. doi: 10.2174/1871526517666170713101734.
Production of Beta-Lactamase enzymes, especially extended- spectrum Beta -Lactamases (ESBL), is a primary mechanism of resistance in these bacteria.The purpose of this study was detection of blaTEM, blaSHV, blaCTXM, blaCTX-M-15, blaPERand blaVEBin K. pneumoniae, isolated from clinical specimens by the PCR method and antibiotic susceptibility patterns in these strains isolated.
During a period from October 2015 to July 2016, 52 K. pneumoniae isolates were collected from general hospitals in the city of Sanandaj, Iran. After identifying the strains by biochemical testing, the disc diffusion method was used for determining antimicrobial susceptibility and screening the ESBL-producing isolates. Detection of blaTEM, blaSHV, blaCTX-M, blaCTX-M-15, blaPER and blaVEBESBL-producing K. pneumoniae was carried out by PCR.
Out of 52-collected K. pneumoniae, highest and lowest rates of resistance related to co-trimoxazole with 67.3 % and amikacin with 30.7 %. 55.7% identified as MDR and 69.23% were ESBL-producing K. pneumoniae.blaSHV was the most prevalent gene in ESBL-producing K. pneumoniae. blaTEM,blaCTX-M,blaCTX-M-15 producing K. pneumoniae strains showed higher rates of drug resistant compared with negative strains (P < 0.05).
Results of this study showed that the prevalence rate of ESBL-producing K. pneumoniae isolates is increasing in MDR strains, which raises concerns regarding the treatment of K. pneumoniae. Therefore, molecular research in the field of antimicrobial resistance of bacteria is essential to prevent the spread of resistant strains.
β-内酰胺酶的产生，尤其是超广谱β-内酰胺酶（ESBL），是这些细菌耐药的主要机制。本研究的目的是通过PCR方法检测从临床标本中分离出的肺炎克雷伯菌中的blaTEM、blaSHV、blaCTXM、blaCTX-M-15、blaPER和blaVEB，并检测这些分离菌株的抗生素敏感性模式。
在2015年10月至2016年7月期间，从伊朗萨南达杰市的综合医院收集了52株肺炎克雷伯菌分离株。通过生化检测鉴定菌株后，采用纸片扩散法测定抗菌药物敏感性并筛选产ESBL的分离株。通过PCR检测产ESBL肺炎克雷伯菌中的blaTEM、blaSHV、blaCTX-M、blaCTX-M-15、blaPER和blaVEB。
在收集的52株肺炎克雷伯菌中，对复方新诺明的耐药率最高，为67.3%，对阿米卡星的耐药率最低，为30.7%。55.7%被鉴定为多重耐药，69.23%为产ESBL的肺炎克雷伯菌。blaSHV是产ESBL肺炎克雷伯菌中最常见的基因。与阴性菌株相比，产blaTEM、blaCTX-M、blaCTX-M-15的肺炎克雷伯菌菌株显示出更高的耐药率（P<0.05）。
本研究结果表明，产ESBL肺炎克雷伯菌分离株在多重耐药菌株中的流行率正在增加，这引发了对肺炎克雷伯菌治疗的担忧。因此，在细菌抗菌药物耐药领域进行分子研究对于防止耐药菌株的传播至关重要。
