Pourmand Mohammad Reza, Hassanzadeh Sepideh, Mashhadi Rahil, Askari Emran
Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Microbiol. 2014 Oct;6(5):341-4.
Methicillin-resistant Staphylococcus aureus (MRSA) is a well-known pathogen with a worldwide distribution. Given the increasing rate of MRSA infections, implementing of reliable, accurate and rapid testing for diagnosis of MRSA is necessary. The aim of this study was to compare four diagnostic methods for detection of MRSA isolates.
From December 2012 to April 2014, 120 S. aureus isolates were collected from three hospitals affiliated with Tehran University of Medical Sciences. MRSA isolates were detected by four different methods including cefoxitin disc diffusion test, oxacillin disc diffusion test, minimum inhibitory concentration (MIC) of oxacillin as determined by MIC test strip, and mecA detection by PCR.
Out of 120 S. aureus isolates, cefoxitin disc diffusion test, oxacillin disc diffusion test and MIC test strip identified 60 (50%), 48 (40%), 55 (45.83%) isolates as MRSA, respectively. The sensitivity and specificity for oxacillin disc diffusion, cefoxitin disc diffusion and MIC of oxacillin were 80% and 100%, 100% and 100%, and 91.6% and 100%, respectively.
Cefoxitin disc diffusion test is reliable substitute for detection of MRSA in clinical laboratory where MIC detection and molecular methods are not accessible.
耐甲氧西林金黄色葡萄球菌（MRSA）是一种广为人知且在全球范围内分布的病原体。鉴于MRSA感染率不断上升，实施可靠、准确且快速的检测方法以诊断MRSA十分必要。本研究旨在比较四种检测MRSA分离株的诊断方法。
2012年12月至2014年4月，从德黑兰医科大学附属的三家医院收集了120株金黄色葡萄球菌分离株。通过四种不同方法检测MRSA分离株，包括头孢西丁纸片扩散法、苯唑西林纸片扩散法、用MIC试纸条测定苯唑西林的最低抑菌浓度（MIC）以及通过聚合酶链反应（PCR）检测mecA。
在120株金黄色葡萄球菌分离株中，头孢西丁纸片扩散法、苯唑西林纸片扩散法和MIC试纸条分别鉴定出60株（50%）、48株（40%）、55株（45.83%）为MRSA。苯唑西林纸片扩散法、头孢西丁纸片扩散法和苯唑西林MIC的敏感性和特异性分别为80%和100%、100%和100%、91.6%和100%。
在无法进行MIC检测和分子方法检测的临床实验室中，头孢西丁纸片扩散法是检测MRSA的可靠替代方法。
