Amini Masoomeh, Pourmand Mohammad Reza, Faridi-Majidi Reza
Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, University of Medical Sciences (TUMS), Tehran, Iran.
Avicenna J Med Biotechnol. 2023 Apr-Jun;15(2):100-107.
Methicillin-resistant (MRSA) has become a worldwide concern as an epidemic bacterium and a cause of nosocomial and community-acquired infections. One of the major problems in the prevention and treatment of infections caused by MRSA strains is their multi-drug resistant trait, which causes the spread of infections and increases the mortality rate. Therefore, a rapid and accurate method is needed to identify MRSA strains, initiate appropriate antibiotic therapy, and control its infection. The aim of this study was to develop a twin lateral flow immunoassay system to detect methicillin-resistant (MRSA).
First, BSA blocked AuNPs-anti-peptidoglycan antibody and AuNPs-anti-BSA antibody were used to detect (). Then, AuNPs-anti-PBP2a antibody was used to specifically detect MRSA. Sensitivity, specificity and limit of detection of this twin immunoassay system were assessed using MRSA, methicillin susceptible and clinical samples. Results were compared to those of cefoxitin disc diffusion (FOX30) and Polymerase Chain Reaction (PCR) as gold standards.
The Limit of Detection (LOD) of this twin system were 10 and 10 CFU/ for the first and second strips, respectively. Sensitivity and specificity of this innovative assay in detecting MRSA were 92.30 and 97.36%, compared to FOX30 and PCR, respectively.
High rates of sensitivity and specificity of this initiative system show its high potentials for rapid and accurate detection of MRSA.
耐甲氧西林金黄色葡萄球菌（MRSA）作为一种流行细菌以及医院获得性感染和社区获得性感染的病原体，已成为全球关注的问题。耐甲氧西林金黄色葡萄球菌菌株引起的感染预防和治疗中的主要问题之一是其多重耐药特性，这导致感染传播并增加死亡率。因此，需要一种快速准确的方法来鉴定耐甲氧西林金黄色葡萄球菌菌株，启动适当的抗生素治疗并控制其感染。本研究的目的是开发一种双侧向免疫分析系统来检测耐甲氧西林金黄色葡萄球菌（MRSA）。
首先，使用牛血清白蛋白封闭的金纳米颗粒-抗肽聚糖抗体和金纳米颗粒-抗牛血清白蛋白抗体进行检测（）。然后，使用金纳米颗粒-抗PBP2a抗体特异性检测耐甲氧西林金黄色葡萄球菌。使用耐甲氧西林金黄色葡萄球菌、甲氧西林敏感菌和临床样本评估该双免疫分析系统的灵敏度、特异性和检测限。将结果与作为金标准的头孢西丁纸片扩散法（FOX30）和聚合酶链反应（PCR）的结果进行比较。
该双系统第一条和第二条试纸条的检测限分别为10和10 CFU/。与FOX30和PCR相比，这种创新检测方法检测耐甲氧西林金黄色葡萄球菌的灵敏度和特异性分别为92.
