Limlomwongse L, Krishnamra N
Department of Physiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
J Nutr Sci Vitaminol (Tokyo). 1989 Aug;35(4):267-77. doi: 10.3177/jnsv.35.267.
To demonstrate different effects of bile and Na taurocholate on calcium absorption, in vivo studies and in situ intestinal loop experiments were performed in intact rats. Only bile (collected from donor rats), but not 15 mM Na taurocholate, significantly increased the jejunal mucosa calcium and 45Ca contents after an intragastric administration of test solution containing 7.5 mM CaCl2 + 45Ca. However, plasma radioactivity which represented lumen to plasma calcium transport was increased by Na taurocholate but not bile, suggesting that both agents enhanced calcium transport across the brush border membrane but in the presence of bile some calcium remained in the mucosal cells. Results from the in situ studies supported the above findings. It was shown that bile and Na taurocholate enhanced the calcium transport from the lumen. However, net absorption was unchanged due to concurrent increase in the efflux of calcium.
为了证明胆汁和牛磺胆酸钠对钙吸收的不同影响，在完整大鼠中进行了体内研究和原位肠袢实验。在胃内给予含有7.5 mM氯化钙 + 45钙的测试溶液后，仅胆汁（从供体大鼠收集）而非15 mM牛磺胆酸钠能显著增加空肠黏膜钙和45钙含量。然而，代表肠腔到血浆钙转运的血浆放射性被牛磺胆酸钠增加，但未被胆汁增加，这表明两种试剂均增强了跨刷状缘膜的钙转运，但在胆汁存在的情况下，一些钙保留在黏膜细胞中。原位研究结果支持上述发现。结果表明，胆汁和牛磺胆酸钠增强了钙从肠腔的转运。然而，由于钙外流同时增加，净吸收没有变化。
