Chalmers Gabhan, McLean John, Hunter D Bruce, Brash Marina, Slavic Durda, Pearl David L, Boerlin Patrick
Department of Pathobiology (Chalmers, McLean, Hunter, Boerlin), Animal Health Laboratory, Laboratory Services Division (Brash, Slavic), and Department of Population Medicine (Pearl), Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1.
Can J Vet Res. 2015 Apr;79(2):129-35.
Pododermatitis is a disease of concern for mink breeders in Canada and worldwide, as it causes discomfort and lowers the breeding rates on farms affected by the disease. Unfortunately, the etiology and pathogenesis of pododermatitis are still unknown. In this study, we compared Staphylococcus spp. and Streptococcus canis isolates from healthy mink with isolates from animals with pododermatitis on 2 farms in Ontario. Almost all hemolytic Staphylococcus spp. isolated were shown to be Staphylococcus delphini Group A by 16S ribosomal ribonucleic acid (rRNA) sequence analysis and polymerase chain reaction (PCR). Pulsed-field gel electrophoresis (PFGE) did not reveal any S. delphini or S. canis clonal lineages specifically associated with pododermatitis, which suggests that these bacteria do not act as primary pathogens, but does not dismiss their potential roles as opportunistic pathogens. While S. delphini and S. canis were the most prevalent bacterial pathogens in mink pododermatitis, they were also present in samples from healthy mink. Arcanobacterium phocae is occasionally isolated from pododermatitis cases, but is difficult to recover with conventional culture methods due to its slow growth. A quantitative real-time PCR was developed for the detection of A. phocae and was tested on 138 samples of footpad tissues from 14 farms. The bacterium was detected only in pododermatitis-endemic farms in Canada and was at higher concentrations in tissues from infected footpads than in healthy tissues. This finding suggests that A. phocae is involved in the pathogenesis of pododermatitis.
足皮炎是加拿大及全球水貂养殖者所关注的一种疾病，因为它会引起不适，并降低受该疾病影响农场的繁殖率。不幸的是，足皮炎的病因和发病机制仍不清楚。在本研究中，我们比较了安大略省两个农场健康水貂与患有足皮炎水貂分离出的葡萄球菌属和犬链球菌。通过16S核糖体核糖核酸（rRNA）序列分析和聚合酶链反应（PCR），几乎所有分离出的溶血性葡萄球菌属都显示为A群海豚葡萄球菌。脉冲场凝胶电泳（PFGE）未揭示任何与足皮炎特别相关的海豚葡萄球菌或犬链球菌克隆谱系，这表明这些细菌并非主要病原体，但并不排除它们作为机会性病原体的潜在作用。虽然海豚葡萄球菌和犬链球菌是水貂足皮炎中最常见的细菌病原体，但它们也存在于健康水貂的样本中。海豹分支杆菌偶尔从足皮炎病例中分离出来，但由于其生长缓慢，用传统培养方法很难回收。我们开发了一种定量实时PCR用于检测海豹分支杆菌，并在来自14个农场的138份脚垫组织样本上进行了测试。该细菌仅在加拿大足皮炎流行的农场中被检测到，并且在受感染脚垫的组织中的浓度高于健康组织。这一发现表明海豹分支杆菌参与了足皮炎的发病机制。
