Stefańska Ilona, Kwiecień Ewelina, Kizerwetter-Świda Magdalena, Chrobak-Chmiel Dorota, Rzewuska Magdalena
Department of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Ciszewskiego 8 St., 02-786 Warsaw, Poland.
Antibiotics (Basel). 2022 Jul 31;11(8):1034. doi: 10.3390/antibiotics11081034.
Growing antimicrobial resistance (AMR) in companion-animal pathogens, including (), is a significant concern for pet treatment as well for public health. Despite the importance of in veterinary and human medicine, studies concerning the AMR of this bacterium are still scarce. A total of 65 strains, isolated from dogs and cats, were assessed to test for susceptibility to six clinically relevant antimicrobials via a microdilution method. The prevalence of the selected acquired-resistance genes was also investigated via PCR. High MIC and MIC values (≥128 μg/mL) were noted for tetracycline, erythromycin and clindamycin. Only a few strains were resistant to the tested beta-lactams (6.2%). Tetracycline resistance was found in 66.2% of the strains. Resistance to erythromycin and clindamycin (ML resistance) was found in 55.4% of the strains. Strains with a phenotype showing concurrent resistance to tetracycline and ML were predominant (53.8%). AMR in the tested strains was associated with a variety of acquired and potentially transferable genes. Tetracycline resistance was conferred by (O) (40.0%), (M) (9.2%), and (T) (1.5%), which is reported for the first time in . In most cases, the (M) gene was detected in relation to the conjugative transposon Tn. The MLS phenotype was confirmed in the strains harboring (B) (43.1%) and (TR) (7.7%). To conclude, a high rate of strains occurring in dogs and cats displayed resistance to antimicrobials important for treatment; moreover, they are a potential reservoirs of various resistance determinants. Therefore, AMR in these pathogens should be continuously monitored, especially regarding the One Health concept.
伴侣动物病原体中不断增加的抗菌药物耐药性（AMR），包括（），不仅是宠物治疗的重大问题，也是公共卫生的重大问题。尽管（）在兽医和人类医学中都很重要，但关于这种细菌抗菌药物耐药性的研究仍然很少。通过微量稀释法，对从狗和猫身上分离出的65株（）菌株进行了评估，以测试它们对六种临床相关抗菌药物的敏感性。还通过聚合酶链反应（PCR）研究了所选获得性耐药基因的流行情况。四环素、红霉素和克林霉素的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）值较高（≥128μg/mL）。只有少数菌株对测试的β-内酰胺类药物耐药（6.2%）。66.2%的菌株对四环素耐药。55.4%的菌株对红霉素和克林霉素耐药（大环内酯-林可酰胺-链阳菌素B耐药，即ML耐药）。同时对四环素和ML耐药的表型菌株占主导地位（53.8%）。测试的（）菌株中的AMR与多种获得性和潜在可转移基因有关。四环素耐药性由（O）（40.0%）、（M）（9.2%）和（T）（1.5%）介导，这是（）首次报道。在大多数情况下，（M）基因与接合转座子Tn相关。在携带（B）（43.1%）和（TR）（7.7%）的菌株中证实了MLS表型。总之，在狗和猫中出现的（）菌株对治疗中重要的抗菌药物有很高的耐药率；此外，它们是各种耐药决定因素的潜在储存库。因此，应持续监测这些病原体中的AMR，特别是考虑到“同一健康”概念。
