Kura Aminu Umar, Saifullah Bullo, Cheah Pike-See, Hussein Mohd Zobir, Azmi Norazrina, Fakurazi Sharida
Laboratory of Vaccines and Immunotherapeutics, Institute of Bioscience, Universiti Putra Malaysia, 43400 Selangor, Malaysia.
Materials Synthesis and Characterization Laboratory, Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Nanoscale Res Lett. 2015 Mar 1;10:105. doi: 10.1186/s11671-015-0742-5. eCollection 2015.
Layered double hydroxide (LDH) is an inorganic-organic nano-layered material that harbours drug between its two-layered sheets, forming a sandwich-like structure. It is attracting a great deal of attention as an alternative drug delivery (nanodelivery) system in the field of pharmacology due to their relative low toxic potential. The production of these nanodelivery systems, aimed at improving human health through decrease toxicity, targeted delivery of the active compound to areas of interest with sustained release ability. In this study, we administered zinc-aluminium-LDH-levodopa nanocomposite (ZAL) and zinc-aluminium nanocomposite (ZA) to Sprague Dawley rats to evaluate for acute oral toxicity following OECD guidelines. The oral administration of ZAL and ZA at a limit dose of 2,000 mg/kg produced neither mortality nor acute toxic signs throughout 14 days of the observation. The percentage of body weight gain of the animals showed no significant difference between control and treatment groups. Animal from the two treated groups gained weight continuously over the study period, which was shown to be significantly higher than the weight at the beginning of the study (P < 0.05). Biochemical analysis of animal serum showed no significant difference between rats treated with ZAL, ZA and controls. There was no gross lesion or histopathological changes observed in vital organs of the rats. The results suggested that ZAL and ZA at 2,000 mg/kg body weight in rats do not induce acute toxicity in the animals. Elemental analysis of tissues of treated animals demonstrated the wider distribution of the nanocomposite including the brain. In summary, findings of acute toxicity tests in this study suggest that zinc-aluminium nanocomposite intercalated with and the un-intercalated were safe when administered orally in animal models for short periods of time. It also highlighted the potential distribution ability of Tween-80 coated nanocomposite after oral administration.
层状双氢氧化物（LDH）是一种无机-有机纳米层状材料，其两层片层之间可容纳药物，形成类似三明治的结构。由于其相对较低的潜在毒性，它作为药理学领域的一种替代药物递送（纳米递送）系统正受到广泛关注。这些纳米递送系统的生产旨在通过降低毒性、将活性化合物靶向递送至感兴趣区域并具有持续释放能力来改善人类健康。在本研究中，我们按照经合组织指南，给斯普拉格-道利大鼠施用锌-铝-LDH-左旋多巴纳米复合材料（ZAL）和锌-铝纳米复合材料（ZA），以评估急性口服毒性。以2000 mg/kg的极限剂量口服ZAL和ZA，在整个14天的观察期内均未产生死亡或急性毒性体征。动物体重增加百分比在对照组和治疗组之间无显著差异。两个治疗组的动物在研究期间体重持续增加，且显著高于研究开始时的体重（P < 0.05）。动物血清的生化分析表明，用ZAL、ZA处理的大鼠与对照组之间无显著差异。在大鼠的重要器官中未观察到肉眼可见的病变或组织病理学变化。结果表明，大鼠体重2000 mg/kg的ZAL和ZA不会在动物中诱发急性毒性。对处理过的动物组织进行元素分析表明，纳米复合材料包括在大脑中的分布更广泛。总之，本研究急性毒性试验的结果表明，在动物模型中短期口服时，插层和未插层的锌-铝纳米复合材料是安全的。它还突出了吐温-80包被的纳米复合材料口服后的潜在分布能力。
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