Saifullah Bullo, Hussein Mohd Zobir, Hussein-Al-Ali Samer Hasan, Arulselvan Palanisamy, Fakurazi Sharida
Materials Synthesis and Characterization Laboratory, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
Drug Des Devel Ther. 2013 Nov 13;7:1365-75. doi: 10.2147/DDDT.S50665. eCollection 2013.
We report the intercalation and characterization of para-amino salicylic acid (PASA) into zinc/aluminum-layered double hydroxides (ZLDHs) by two methods, direct and indirect, to form nanocomposites: PASA nanocomposite prepared by a direct method (PASA-D) and PASA nanocomposite prepared by an indirect method (PASA-I). Powder X-ray diffraction, Fourier-transform infrared spectroscopy, and thermogravimetric analysis revealed that the PASA drugs were accommodated within the ZLDH interlayers. The anions of the drug were accommodated as an alternate monolayer (along the long-axis orientation) between ZLDH interlayers. Drug loading was estimated to be 22.8% and 16.6% for PASA-D and PASA-I, respectively. The in vitro release properties of the drug were investigated in physiological simulated phosphate-buffered saline solution of pH 7.4 and 4.8. The release followed the pseudo-second-order model for both nanocomposites. Cell viability (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide [MTT] assays) was assessed against normal human lung fibroblast MRC-5 and 3T3 mouse fibroblast cells at 24, 48, and 72 hours. The results showed that the nanocomposite formulations did not possess any cytotoxicity, at least up to 72 hours.
我们报告了通过直接和间接两种方法将对氨基水杨酸（PASA）插层到锌/铝层状双氢氧化物（ZLDHs）中以形成纳米复合材料的过程及表征：通过直接法制备的PASA纳米复合材料（PASA-D）和通过间接法制备的PASA纳米复合材料（PASA-I）。粉末X射线衍射、傅里叶变换红外光谱和热重分析表明，PASA药物被容纳在ZLDH的层间。药物阴离子以交替单层（沿长轴方向）的形式容纳在ZLDH层间。PASA-D和PASA-I的载药量分别估计为22.8%和16.6%。在pH值为7.4和4.8的生理模拟磷酸盐缓冲盐溶液中研究了药物的体外释放特性。两种纳米复合材料的释放均遵循准二级模型。在24、48和72小时时，针对正常人肺成纤维细胞MRC-5和3T3小鼠成纤维细胞评估了细胞活力（3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四氮唑溴盐[MTT]测定）。结果表明，纳米复合制剂至少在72小时内不具有任何细胞毒性。
