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用于增强shRNA介导的抗癌效果的鱼精蛋白纳米颗粒

Protamine nanoparticles for improving shRNA-mediated anti-cancer effects.

作者信息

Liu Ming, Feng Bo, Shi Yijie, Su Chang, Song Huijuan, Cheng Wei, Zhao Liang

机构信息

School of Pharmacy, Liaoning Medical University, Jinzhou, 121000 People's Republic of China.

School of Veterinary Medicine, Liaoning Medical University, Jinzhou, 121000 People's Republic of China.

出版信息

Nanoscale Res Lett. 2015 Mar 19;10:134. doi: 10.1186/s11671-015-0845-z. eCollection 2015.

DOI:10.1186/s11671-015-0845-z
PMID:25852425
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4385308/
Abstract

Protamine nanoparticles were designed by encapsulating small hairpin RNA (shRNA)-expressing plasmid DNA targeting the Bcl-2 gene (shBcl-2) to silence apoptosis-related Bcl-2 protein for improving the transfection efficiency and cytotoxicity in cancer therapy. Our findings demonstrated that the obtained protamine nanoparticles possessed excellent characterizations of small particle size, homogenous distribution, positive charge, and high encapsulation efficiency of gene. shBcl-2 loaded in nanoparticles (NPs) was protected effectively from the degradation of DNase I and serum. More importantly, it significantly improved the efficiency of transfection of shRNA in vitro in A549 cells and increased its cytotoxicity and induced more cell apoptosis by silencing Bcl-2.

摘要

通过包裹靶向Bcl-2基因的小发夹RNA（shRNA）表达质粒DNA（shBcl-2）来设计鱼精蛋白纳米颗粒，以沉默凋亡相关的Bcl-2蛋白，从而提高癌症治疗中的转染效率和细胞毒性。我们的研究结果表明，所获得的鱼精蛋白纳米颗粒具有粒径小、分布均匀、带正电荷和基因包封效率高的优异特性。负载于纳米颗粒（NPs）中的shBcl-2能有效抵御DNase I和血清的降解。更重要的是，它显著提高了shRNA在体外对A549细胞的转染效率，增强了其细胞毒性，并通过沉默Bcl-2诱导更多细胞凋亡。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3706/4385308/fd9c579594bf/11671_2015_845_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3706/4385308/8bca8501e8f7/11671_2015_845_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3706/4385308/3832b69b7717/11671_2015_845_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3706/4385308/ab54ec1247dd/11671_2015_845_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3706/4385308/c523109f2ada/11671_2015_845_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3706/4385308/fd9c579594bf/11671_2015_845_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3706/4385308/8bca8501e8f7/11671_2015_845_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3706/4385308/3832b69b7717/11671_2015_845_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3706/4385308/ab54ec1247dd/11671_2015_845_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3706/4385308/c523109f2ada/11671_2015_845_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3706/4385308/fd9c579594bf/11671_2015_845_Fig5_HTML.jpg

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