Zhang Yanjie, Hu Zongli, Zhu Mingku, Zhu Zhiguo, Wang Zhijin, Tian Shibing, Chen Guoping
†Bioengineering College, Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Chongqing University, Campus B, Room 515, 174 Shapingba Main Street, Chongqing 400044, People's Republic of China.
‡The Institute of Vegetable Research, Chongqing Academy of Agricultural Sciences, 401329 Chongqing, People's Republic of China.
J Agric Food Chem. 2015 Apr 29;63(16):4160-9. doi: 10.1021/acs.jafc.5b00473. Epub 2015 Apr 15.
Kohlrabi (Brassica oleracea var. gongylodes L.) is an important dietary vegetable cultivated and consumed widely for the round swollen stem. Purple kohlrabi shows abundant anthocyanin accumulation in the leaf and swollen stem. Here, different kinds of anthocyanins were separated and identified from the purple kohlrabi cultivar (Kolibri) by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry. In order to study the molecular mechanism of anthocyanin biosynthesis in purple kohlrabi, the expression of anthocyanin biosynthetic genes and regulatory genes in purple kohlrabi and a green cultivar (Winner) was examined by quantitative PCR. In comparison with the colorless parts in the two cultivars, most of the anthocyanin biosynthetic genes and two transcription factors were drastically upregulated in the purple tissues. To study the effects of light shed on the anthocyanin accumulation of kohlrabi, total anthocyanin contents and transcripts of associated genes were analyzed in sprouts of both cultivars grown under light and dark conditions.
球茎甘蓝（Brassica oleracea var. gongylodes L.）是一种重要的食用蔬菜，因其圆形肿胀的茎而被广泛种植和食用。紫色球茎甘蓝在叶片和肿胀的茎中积累了大量花青素。在此，通过高效液相色谱-电喷雾电离串联质谱法从紫色球茎甘蓝品种（Kolibri）中分离并鉴定了不同种类的花青素。为了研究紫色球茎甘蓝中花青素生物合成的分子机制，通过定量PCR检测了紫色球茎甘蓝和绿色品种（Winner）中花青素生物合成基因和调控基因的表达。与两个品种的无色部分相比，大多数花青素生物合成基因和两个转录因子在紫色组织中显著上调。为了研究光照对球茎甘蓝花青素积累的影响，分析了在光照和黑暗条件下生长的两个品种芽苗中总花青素含量和相关基因的转录本。
