Sariya Ladawan, Kladmanee Kan, Bhusri Benjaporn, Thaijongrak Prawporn, Tonchiangsai Kanittha, Chaichoun Kridsada, Ratanakorn Parntep
Jpn J Vet Res. 2015 Feb;63(1):5-14.
Chlamydiosis, caused by Chlamydiaceae, is a zoonotic disease found in humans and several species of animals, including reptiles and amphibians. Although chlamydiosis in saltwater crocodiles has been previously reported in South Africa and Papua New Guinea, the reported strains have not been identified or confirmed. Therefore, the main aim of this study was to sequence and characterize Chamydiaceae isolated from Siamese crocodiles. Results showed the 16S ribosomal (r) RNA and the 16S/23S rRNA gene of the crocodile isolates were closely related to the genus Chlamydophila with matched identity greater than 98%. The phylogenetic tree constructed from the 16S/23S rRNA gene showed the crocodile cluster diverges far from Cp. caviae with a 100% bootstrap value. The tree based on the ompA gene loci distinguished the crocodile strains into genotypes I, II, and III. The present study is the first report on Chlamydophila detected in Siamese crocodiles that is genetically distinct from the known species of Chlamydiaceae.
由衣原体科引起的衣原体病是一种人畜共患病，可在人类和包括爬行动物及两栖动物在内的多种动物物种中发现。尽管此前在南非和巴布亚新几内亚已报告过咸水鳄的衣原体病，但所报告的菌株尚未得到鉴定或确认。因此，本研究的主要目的是对从暹罗鳄分离出的衣原体科进行测序和特征分析。结果显示，鳄鱼分离株的16S核糖体（r）RNA和16S/23S rRNA基因与嗜衣原体属密切相关，匹配同一性大于98%。根据16S/23S rRNA基因构建的系统发育树显示，鳄鱼聚类与嗜肺衣原体相距甚远，自展值为100%。基于ompA基因座的树将鳄鱼菌株分为基因型I、II和III。本研究是关于在暹罗鳄中检测到的嗜衣原体的首次报告，该嗜衣原体在基因上与已知的衣原体科物种不同。
