The Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, Thailand.
Center of Excellence in Systems Biology, Research Affairs, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
PLoS One. 2021 May 27;16(5):e0252081. doi: 10.1371/journal.pone.0252081. eCollection 2021.
Chlamydia is a known pathogen in both saltwater and freshwater crocodiles. However, the exact species/strain has not been clearly identified. In this study, we successfully cultivated Siamese crocodile Chlamydia in McCoy cells at a temperature of 30°C. Electron microscopy; phylogeny based on nine conserved taxonomically informative markers, on ompA, or on seven housekeeping genes; and whole-genome sequencing and analysis of the isolate confirmed the identity of the isolate as a new member of the genus Chlamydia, a new species that we name Chlamydia crocodili.
在咸水鳄和淡水鳄中,衣原体都是一种已知的病原体。但是,确切的物种/菌株尚未明确确定。在这项研究中,我们成功地在 30°C 的温度下在 McCoy 细胞中培养了暹罗鳄衣原体。电镜观察;基于九个保守的分类学信息标记、ompA 或七个管家基因的系统发育;以及分离株的全基因组测序和分析证实了分离株是衣原体属的一个新成员,一种新的衣原体物种,我们将其命名为鳄鱼衣原体。