Dey Avijit, Dutta Narayan, Pattanaik Ashok Kumar, Sharma Kusumakar
Jpn J Vet Res. 2015 Feb;63(1):15-24.
To study the effect of supplementation of tanniferous tree leaves Ficus infectoria on antioxidant status and immune response, twenty four lambs were randomly divided into four groups of six each in a completely randomized design and fed either a conventional supplement (CON) or experimental supplements (FILM-I, FILM-II and FILM-III) containing 1.0, 1.5 and 2.0% condensed tannins (CT), respectively by replacement of wheat bran of supplement CON with Ficus infectoria leaf meal (FILM). Blood biochemical profile was monitored in all lambs at 0, 45, 90, 135, 180 days of feeding. Although haemato-biochemical parameters remained similar, there was significant (p < 0.05) improvement in catalase activity, total thiol and protein thiol groups with reduction in lipid peroxidation (LPO) in lambs fed FILM diet irrespective of levels. However, intracellular status of reduced glutathione, and superoxide dismutase activity was improved (p < 0.05) only in FILM-II and FILM-III supplemented lambs. The cell-mediated immune response was significantly (p < 0.05) improved in all the lambs fed FILM supplemented diets. Improved antioxidant status and immunity in FILM supplemented lambs increased voluntary feed intake irrespective of level. However, the average daily gain for a period of 180 d showed a significant (p < 0.05) increase by the supplementation of FILM-II diet containing 1.5% CT. The present study reveals that the supplementation of Ficus infectoria leaf meal up to 21.2% in the concentrate mixture could improve the antioxidant status and immunity in lambs. However, as feed efficiency was reduced at higher levels due to presence of CT, 15.9% supplementation containing 1.5% condensed tannins in concentrate mixture is suggested to improve the health and production performance of lambs.
为研究添加含单宁的榕树树叶对羔羊抗氧化状态和免疫反应的影响，将24只羔羊按完全随机设计随机分为4组，每组6只，分别饲喂常规补充料（CON）或分别含有1.0%、1.5%和2.0%缩合单宁（CT）的实验补充料（FILM-I、FILM-II和FILM-III），用榕树叶粉（FILM）替代CON补充料中的麦麸。在饲喂的第0、45、90、135、180天对所有羔羊的血液生化指标进行监测。尽管血液生化参数保持相似，但无论水平如何，饲喂FILM日粮的羔羊过氧化氢酶活性、总巯基和蛋白质巯基显著提高（p<0.05），脂质过氧化（LPO）降低。然而，仅在补充FILM-II和FILM-III的羔羊中，还原型谷胱甘肽的细胞内状态和超氧化物歧化酶活性得到改善（p<0.05）。所有饲喂FILM补充日粮的羔羊的细胞介导免疫反应均显著改善（p<0.05）。补充FILM的羔羊抗氧化状态和免疫力的改善增加了自愿采食量，与水平无关。然而，含1.5%CT的FILM-II日粮补充后，180天的平均日增重显著增加（p<0.05）。本研究表明，在精料混合料中添加高达21.2%的榕树叶粉可改善羔羊的抗氧化状态和免疫力。然而，由于CT的存在，较高水平下饲料效率降低，建议在精料混合料中添加15.9%、含1.5%缩合单宁的饲料，以改善羔羊的健康和生产性能。
