4'-硫代脱氧核糖核酸模板在体外和哺乳动物细胞中向天然核糖核酸的转录。
Transcription of 4'-thioDNA templates to natural RNA in vitro and in mammalian cells.
作者信息
Maruyama Hideto, Furukawa Kazuhiro, Kamiya Hiroyuki, Minakawa Noriaki, Matsuda Akira
机构信息
Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12, Nishi-6, Kita-ku, Sapporo 060-0812, Japan.
出版信息
Chem Commun (Camb). 2015 May 7;51(37):7887-90. doi: 10.1039/c4cc08862j.
Synthetic chemically modified nucleic acids, which are compatible with DNA/RNA polymerases, have great potential as a genetic material for synthetic biological studies. A comprehensive analysis of the transcription of 4'-thioDNA templates to natural RNA is herein reported. The modifications of DNA with 2'-deoxy-4'-thionucleosides, so-called 4'-thioDNA, were found to act as templates for transcription with RNA polymerases both in vitro and in mammalian cells.
与DNA/RNA聚合酶兼容的合成化学修饰核酸作为合成生物学研究的遗传物质具有巨大潜力。本文报道了对4'-硫代DNA模板转录为天然RNA的全面分析。发现用2'-脱氧-4'-硫代核苷修饰的DNA，即所谓的4'-硫代DNA，在体外和哺乳动物细胞中均可作为RNA聚合酶转录的模板。
Zotero 插件