Abdull Razis Ahmad Faizal, Konsue Nattaya, Ioannides Costas
Food Safety Research Centre (FOSREC), Faculty of Food Science and Technology, Universiti Putra Malaysia, UPM Serdang, Selangor, Malaysia E-mail :
Asian Pac J Cancer Prev. 2015;16(7):2679-83. doi: 10.7314/apjcp.2015.16.7.2679.
Phenethyl isothiocyanate (PEITC), the most comprehensively studied aromatic isothiocyanate, has been shown to act as an anti-cancer agent mainly through modulation of biotransformation enzymes responsible for metabolizing carcinogens in the human body. Humans are often exposed to carcinogenic factors, some of which through the diet, such as polycyclic aromatic hydrocarbon benzo[a]pyrene via the consumption of over-cooked meats. Inhibition of the enzymes responsible for the bioactivation of this carcinogen, for example CYP1A1, the major enzyme required for polycyclic aromatic hydrocarbons (PAHs) bioactivation, is recognized as a chemoprevention strategy.
To evaluate the inhibitory effects of PEITC against benzo[a]pyrene-induced rise in rat liver CYP1A1 mRNA and apoprotein levels.
Precision cut rat liver slices were treated with benzo[a]pyrene at 1 and 5 μM in the presence of PEITC (1-25 μM) for 24 hours, followed by determination of CYP1A1 mRNA and apoprotein levels using quantitative polymerase chain reaction and immunoblotting.
Findings revealed that PEITC inhibited benzo[a]pyrene-induced rise in rat liver CYP1A1 mRNA in a dose-dependent manner as well as the apoprotein levels of CYP1A.
It was demonstrated that PEITC can directly inhibit the bioactivation of benzo[a]pyrene, indicating chemopreventive potential.
苯乙基异硫氰酸酯（PEITC）是研究最为全面的芳香族异硫氰酸酯，已显示其作为抗癌剂主要是通过调节负责代谢人体致癌物的生物转化酶来发挥作用。人类经常接触致癌因素，其中一些通过饮食接触，例如通过食用过度烹饪的肉类接触多环芳烃苯并[a]芘。抑制负责这种致癌物生物活化的酶，例如细胞色素P450 1A1（CYP1A1），多环芳烃（PAHs）生物活化所需的主要酶，被认为是一种化学预防策略。
评估PEITC对苯并[a]芘诱导的大鼠肝脏CYP1A1 mRNA和载脂蛋白水平升高的抑制作用。
将精密切割的大鼠肝脏切片在存在PEITC（1 - 25 μM）的情况下用1和5 μM的苯并[a]芘处理24小时，然后使用定量聚合酶链反应和免疫印迹法测定CYP1A1 mRNA和载脂蛋白水平。
研究结果显示，PEITC以剂量依赖性方式抑制苯并[a]芘诱导的大鼠肝脏CYP1A1 mRNA以及CYP1A的载脂蛋白水平升高。
证明PEITC可直接抑制苯并[a]芘的生物活化，表明其具有化学预防潜力。
