Liu Yueqin, Lin Xin, Bao Tinjun, Ni Ping, Xie Chanjuan, Shen Huiling, Xu Wenlin, Xu Huaxi, Su Zhaoliang
Department of Oncology, Fourth People's Hospital Affiliated to Jiangsu University, Zhenjiang 212001, China.
Institute of Immunology, School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang 212013, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2015 Apr;31(4):467-9, 473.
To detect the levels of miR-21a-5p, miR-155-5p, miR-218-5p, miR-222-3p, miR-494-3p in ovarian cancer tissues and the number of myeloid-derived suppressor cells (MDSCs) in peripheral blood and spleen in ovarian cancer-bearing mice, and explore their clinical significance and correlations.
The mRNA expressions of miR-21a-5p, miR-155a-5p, miR-218-5p, miR-222-3p, miR-494-3p were detected by real-time quantitative PCR (qRT-PCR) in tumor tissues and tumor-adjacent normal tissues from 12 ovarian cancer-bearing mice. The frequency of MDSCs in the peripheral blood and spleen from the 12 tumor-bearing mice was measured by flow cytometry. Spearman correlation analysis was used to find out the correlations between MDSCs and miRNAs.
Compared with the normal mice, the number of MDSCs in the peripheral blood and spleen of the tumor-bearing mice significantly increased. The levels of miR-21a-5p, miR-218-5p and miR-222-3p in tumor tissues were significantly higher than those in tumor-adjacent normal tissues; conversely, miR-155a-5p and miR-494-3p levels in the former were significantly lower than those in the latter. There was no correlation between miR-222-3p and MDSCs, but miR-494-3p had a negative correlation with MDSCs. The expression differences of miR-21a-5p, miR-155a-5p, miR-218-5p between tumor tissues and tumor-adjacent normal tissues had positive correlations with the number of MDSCs.
The levels of miR-21a-5p, miR-155a-5p, miR-218-5p, miR-494-3p in tumor tissues had correlations with the number of MDSCs in the peripheral blood and spleen of ovarian cancer-bearing mice.
检测卵巢癌组织中miR-21a-5p、miR-155-5p、miR-218-5p、miR-222-3p、miR-494-3p的水平以及荷卵巢癌小鼠外周血和脾脏中髓源性抑制细胞（MDSCs）的数量，并探讨其临床意义及相关性。
采用实时定量PCR（qRT-PCR）检测12只荷卵巢癌小鼠肿瘤组织及肿瘤旁正常组织中miR-21a-5p、miR-155a-5p、miR-218-5p、miR-222-3p、miR-494-3p的mRNA表达。通过流式细胞术检测12只荷瘤小鼠外周血和脾脏中MDSCs的频率。采用Spearman相关性分析来找出MDSCs与miRNAs之间的相关性。
与正常小鼠相比，荷瘤小鼠外周血和脾脏中MDSCs的数量显著增加。肿瘤组织中miR-21a-5p、miR-218-5p和miR-222-3p的水平显著高于肿瘤旁正常组织；相反，前者中miR-155a-5p和miR-494-3p的水平显著低于后者。miR-222-3p与MDSCs之间无相关性，但miR-494-3p与MDSCs呈负相关。肿瘤组织与肿瘤旁正常组织之间miR-21a-5p、miR-155a-5p、miR-218-5p的表达差异与MDSCs的数量呈正相关。
肿瘤组织中miR-21a-5p、miR-155a-5p、miR-218-5p、miR-494-3p的水平与荷卵巢癌小鼠外周血和脾脏中MDSCs的数量相关。
