Garino Cristiano, De Paolis Angelo, Coïsson Jean Daniel, Arlorio Marco
Dipartimento di Scienze del Farmaco & Drug and Food Biotechnology (DFB) Center, Università del Piemonte Orientale "A. Avogadro", largo Donegani 2, 28100 Novara, Italy.
Istituto di Scienze delle Produzioni Alimentari (ISPA) - CNR, via Prov.le Lecce - Monteroni, 73100 Lecce, Italy.
Comput Biol Chem. 2015 Jun;56:30-2. doi: 10.1016/j.compbiolchem.2015.03.004. Epub 2015 Mar 30.
A short partial sequence of 28 amino acids is all the information we have so far about the putative allergen 2S albumin from almond. The aim of this work was to analyze this information using mainly bioinformatics tools, in order to verify its rightness. Based on the results reported in the paper describing this allergen from almond, we analyzed the original data of amino acids sequencing through available software. The degree of homology of the almond 12kDa protein with any other known 2S albumin appears to be much lower than the one reported in the paper that firstly described it. In a publicly available cDNA library we discovered an expressed sequence tag which translation generates a protein that perfectly matches both of the sequencing outputs described in the same paper. A further analysis indicated that the latter protein seems to belong to the vicilin superfamily rather than to the prolamin one. The fact that also vicilins are seed storage proteins known to be highly allergenic would explain the IgE reactivity originally observed. Based on our observations we suggest that the IgE reactive 12kDa protein from almond currently known as Pru du 2S albumin is in reality the cleaved N-terminal region of a 7S vicilin like protein.
到目前为止，我们所掌握的关于杏仁假定过敏原2S白蛋白的全部信息，只是一段由28个氨基酸组成的短序列。这项工作的目的是主要使用生物信息学工具来分析这些信息，以验证其正确性。基于描述杏仁中这种过敏原的论文所报道的结果，我们通过可用软件分析了氨基酸测序的原始数据。杏仁12kDa蛋白与任何其他已知2S白蛋白的同源程度，似乎比首次描述它的论文中所报道的要低得多。在一个公开可用的cDNA文库中，我们发现了一个表达序列标签，其翻译产生的一种蛋白质与同一篇论文中描述的两个测序结果完全匹配。进一步分析表明，后一种蛋白质似乎属于豌豆球蛋白超家族，而不是醇溶蛋白家族。豌豆球蛋白也是已知具有高度致敏性的种子储存蛋白，这一事实可以解释最初观察到的IgE反应性。基于我们的观察结果，我们认为目前被称为Pru du 2S白蛋白的杏仁中IgE反应性12kDa蛋白，实际上是一种7S豌豆球蛋白样蛋白的N端裂解区域。
