扁桃（甜扁桃）球蛋白鉴定为食物过敏原。
Identification of Almond ( Prunus dulcis) Vicilin As a Food Allergen.
机构信息
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering , China Agricultural University , No. 17 Qinghua Donglu, Haidian District , Beijing 100038 , P. R. China.
Agricultural Research Service, Pacific West Area, Western Regional Research Center , U. S. Department of Agriculture , 800 Buchanan Street , Albany , California 94710 , United States.
出版信息
J Agric Food Chem. 2019 Jan 9;67(1):425-432. doi: 10.1021/acs.jafc.8b05290. Epub 2018 Dec 20.
Almond is one of the tree nuts listed by U.S. FDA as a food allergen source. A food allergen identified with patient sera has been debated to be the 2S albumin or the 7S vicilin. However, neither of these proteins has been defined as a food allergen. The purpose of this study was to clone, express, and purify almond vicilin and test whether it is a food allergen. Western blot experiment was performed with 18 individual sera from patients with double-blind, placebo-controlled clinical almond allergy. The results showed that 44% of the sera contained IgE antibodies that recognized the recombinant almond vicilin, indicating that it is an almond allergen. Identifying this and additional almond allergens will facilitate the understanding of the allergenicity of seed proteins in tree nuts and their cross-reactivity.
杏仁是美国食品药品监督管理局（FDA）列为食物过敏原来源的树坚果之一。与患者血清发生反应的食物过敏原一直存在争议，有人认为是 2S 白蛋白，也有人认为是 7S 豆球蛋白。然而，这两种蛋白都尚未被确认为食物过敏原。本研究的目的是克隆、表达和纯化杏仁豆球蛋白，并验证其是否为食物过敏原。采用 18 份来自经双盲、安慰剂对照临床试验确诊的杏仁过敏患者的血清进行 Western blot 实验。结果显示，44%的血清含有可识别重组杏仁豆球蛋白的 IgE 抗体，表明其为杏仁过敏原。鉴定出这种及其他杏仁过敏原，将有助于理解树坚果中种子蛋白的致敏性及其交叉反应性。
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