猪繁殖与呼吸综合征病毒基质蛋白中的单个氨基酸缺失赋予其对具有广泛中和活性的多克隆猪抗体的抗性。
A single amino acid deletion in the matrix protein of porcine reproductive and respiratory syndrome virus confers resistance to a polyclonal swine antibody with broadly neutralizing activity.
作者信息
Trible Benjamin R, Popescu Luca N, Monday Nicholas, Calvert Jay G, Rowland Raymond R R
机构信息
Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, Kansas, USA
Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, Kansas, USA.
出版信息
J Virol. 2015 Jun;89(12):6515-20. doi: 10.1128/JVI.03287-14. Epub 2015 Apr 8.
Abstract
Assessment of virus neutralization (VN) activity in 176 pigs infected with porcine reproductive and respiratory syndrome virus (PRRSV) identified one pig with broadly neutralizing activity. A Tyr-10 deletion in the matrix protein provided escape from broad neutralization without affecting homologous neutralizing activity. The role of the Tyr-10 deletion was confirmed through an infectious clone with a Tyr-10 deletion. The results demonstrate differences in the properties and specificities of VN responses elicited during PRRSV infection.
摘要
对176头感染猪繁殖与呼吸综合征病毒（PRRSV）的猪的病毒中和（VN）活性进行评估后，发现有一头猪具有广泛中和活性。基质蛋白中的酪氨酸-10缺失使病毒逃避了广泛中和，同时又不影响同源中和活性。通过带有酪氨酸-10缺失的感染性克隆证实了酪氨酸-10缺失的作用。结果表明，PRRSV感染期间引发的VN反应在特性和特异性上存在差异。
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