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基于细胞色素氧化酶亚基I(COI)和小亚基核糖体核糖核酸(SSU rRNA)基因进一步解析黏菌纲(黏菌)的系统发育。

Further resolving the phylogeny of Myxogastria (slime molds) based on COI and SSU rRNA genes.

作者信息

Liu Q Sh, Yan Sh Zh, Chen Sh L

出版信息

Genetika. 2015 Jan;51(1):46-53. doi: 10.7868/s0016675814110071.

DOI:10.7868/s0016675814110071
PMID:25857192
Abstract

To date, molecular systematics of Myxogastria has been based primarily on small subunit ribosomal RNA (SSU rRNA) and elongation factor 1-alpha (EF-1α) genes. To establish a natural classification system for the organisms, we examined phylogenetic relationships among myxogastrian species using cytochrome c oxidase subunit I (COL) and SSU rRNA genes. Twenty new sequences were obtained, including 10 COI and 10 SSU rRNA sequences, were compared with sequences of related species from GenBank in order to construct phylogenic trees. The analysis of the two data sets supported the modern phylogeny of myxogastria: orders Liceida and Trichiida formed a sister group at the most basal clade, while orders Stemonitida and Physarida formed a close group, and order Echinostelida was a sister group to Stemonitida and Physarida. However, the partial COI sequences were too conserved to resolve of the branches in Stemonitida and Physarida. In addition, we also deemed the specific edited mRNA events of COI sequences in myxogastrian species.

摘要

迄今为止,黏菌纲的分子系统学主要基于小亚基核糖体RNA(SSU rRNA)和延伸因子1-α(EF-1α)基因。为了建立该生物的自然分类系统,我们使用细胞色素c氧化酶亚基I(COL)和SSU rRNA基因研究了黏菌纲物种之间的系统发育关系。获得了20个新序列,包括10个COI和10个SSU rRNA序列,并与来自GenBank的相关物种序列进行比较,以构建系统发育树。对这两个数据集的分析支持了黏菌纲的现代系统发育:虱黏菌目和绒泡黏菌目在最基部的分支形成姐妹群,而发网黏菌目和原质黏菌目形成一个紧密的类群,刺丝黏菌目是发网黏菌目和原质黏菌目的姐妹群。然而,部分COI序列过于保守,无法解析发网黏菌目和原质黏菌类群中的分支。此外,我们还研究了黏菌纲物种中COI序列的特定编辑mRNA事件。

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