Vinogradova A P, Lebedeva M A, Lutova L A
Genetika. 2015 Jan;51(1):54-62.
It is known that two key groups of plant hormones--auxins and cytokinins--play an important role in plant tumor development. The formation of Agrobacterium-induced tumors results from the horizontal transfer of bacterial oncogenes involved in the biosynthesis of these hormones in the plant genome. The role of transcriptional factors in plant tumor development is poorly investigated. It can be assumed that tumor development associated with abnormal cell proliferation can be controlled by the same set of transcription factors that control normal cell proliferation and, in particular, transcription factors that regulate meristem activity. In the present study, we analyzed the histological organization and distribution of proliferating cells in tumors induced by Agrobacterium tumefaciens on pea hypocotyls. In addition, the expression of a set of meristem-specific genes with Agrobacterium tumefaciens-induced tumor development was analyzed. In general, our results indicate that meristematic structures are present in A. tumefaciens-induced tumors and that the development of such tumors is associated with increased expression of a key gene regulating the root apical meristem--the WOX5 gene.
已知两类关键的植物激素——生长素和细胞分裂素——在植物肿瘤发育中起重要作用。根癌农杆菌诱导的肿瘤形成是由于参与这些激素生物合成的细菌致癌基因在植物基因组中的横向转移。转录因子在植物肿瘤发育中的作用研究较少。可以推测，与异常细胞增殖相关的肿瘤发育可能由控制正常细胞增殖的同一组转录因子控制，特别是调节分生组织活性的转录因子。在本研究中，我们分析了根癌农杆菌诱导豌豆下胚轴肿瘤的组织学结构和增殖细胞的分布。此外，还分析了一组分生组织特异性基因在根癌农杆菌诱导肿瘤发育过程中的表达。总体而言，我们的结果表明，根癌农杆菌诱导的肿瘤中存在分生组织结构，并且此类肿瘤的发育与调节根尖分生组织的关键基因——WOX5基因的表达增加有关。
