Watanabe M, Suzuki K
RI Research Center, School of Med., Yokohama City Univ.
Gan No Rinsho. 1989 Oct;35(13):1512-6.
We investigated the heat-sensitivities of human normal and cancer cells at different growth conditions in vitro. We found no difference in sensitivities between normal and cancer cells at growing condition. Normal cells at confluence, however, reduced their heat-sensitivity 5-6 times than at growing condition, while the cancer cells did not. Analysis by monoclonal antibodies for hsp 70 showed the differential staining patterns between normal and cancer cells after treatment with heat (43 degrees C for 2 hours). The constitutive expression levels of hsp 70 in cancer cells were 2-3 times higher than those in normal cells. However, the degrees of hsp 70 induction by heat shock treatment in cancer cells were lower than those in normal cells. These results suggest that cancer cells may be abnormal in expression mechanisms of hsp 70.
我们研究了体外不同生长条件下人正常细胞和癌细胞的热敏感性。我们发现正常细胞和癌细胞在生长条件下的敏感性没有差异。然而，汇合状态下的正常细胞其热敏感性比生长状态下降低了5 - 6倍，而癌细胞则没有。用针对hsp 70的单克隆抗体进行分析表明，热处理（43℃，2小时）后正常细胞和癌细胞呈现出不同的染色模式。癌细胞中hsp 70的组成型表达水平比正常细胞高2 - 3倍。然而，癌细胞经热休克处理后hsp 70的诱导程度低于正常细胞。这些结果表明癌细胞在hsp 70的表达机制上可能存在异常。
