[富血小板纤维蛋白对成骨细胞生物学特性的影响]
[The effect of platelet-rich fibrin on biologic characteristics of osteoblasts].
作者信息
Sun Xiao-Lin, Zhou Yan-Min, Zhao Jing-Hui, Zheng Ling, Yang Ting-Ting
机构信息
Department of Oral implantation, Jilin University Affiliated Stomatology Hospital. Changchun 130021, Jilin Province, China.E-mail:
出版信息
Shanghai Kou Qiang Yi Xue. 2015 Feb;24(1):61-4.
PURPOSE
To investigate the effect of platelet-rich fibrin on biologic characteristics of osteoblasts.
METHODS
Osteoblasts were cultured with or without a PRF membrane. The cell proliferation was detected by MTT. The alkaline phosphatase (ALP) stain and the positive expression of collagen type I, osteoprotegerin and RANKL were measured. The data was statistically analyzed with SPSS17.0 software package.
RESULTS
PRF promoted cell proliferation and increased expression of ALP, collagen typeI and OPG significantly, but there was no significant impact on expression of RANKL.
CONCLUSIONS
PRF promotes osteoblasts proliferation, differentiation and OPG expression. PRF is involved in bone remodeling via regulating expression of OPG and RANKL.
目的
探讨富血小板纤维蛋白对成骨细胞生物学特性的影响。
方法
在有或无PRF膜的条件下培养成骨细胞。采用MTT法检测细胞增殖情况。检测碱性磷酸酶（ALP）染色及Ⅰ型胶原、骨保护素和RANKL的阳性表达。数据采用SPSS17.0软件包进行统计学分析。
结果
PRF显著促进细胞增殖，增加ALP、Ⅰ型胶原和OPG的表达，但对RANKL的表达无显著影响。
结论
PRF促进成骨细胞增殖、分化及OPG表达。PRF通过调节OPG和RANKL的表达参与骨重塑。
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