a Department of Oral Implantology , University of Wuhan , Wuhan, China.
b Pain Clinic , Nice , France.
Platelets. 2018 Jan;29(1):48-55. doi: 10.1080/09537104.2017.1293807. Epub 2017 Mar 29.
Platelet-rich plasma (PRP) has been utilized for many years as a regenerative agent capable of inducing vascularization of various tissues using blood-derived growth factors. Despite this, drawbacks mostly related to the additional use of anti-coagulants found in PRP have been shown to inhibit the wound healing process. For these reasons, a novel platelet concentrate has recently been developed with no additives by utilizing lower centrifugation speeds. The purpose of this study was therefore to investigate osteoblast behavior of this novel therapy (injectable-platelet-rich fibrin; i-PRF, 100% natural with no additives) when compared to traditional PRP. Human primary osteoblasts were cultured with either i-PRF or PRP and compared to control tissue culture plastic. A live/dead assay, migration assay as well as a cell adhesion/proliferation assay were investigated. Furthermore, osteoblast differentiation was assessed by alkaline phosphatase (ALP), alizarin red and osteocalcin staining, as well as real-time PCR for genes encoding Runx2, ALP, collagen1 and osteocalcin. The results showed that all cells had high survival rates throughout the entire study period irrespective of culture-conditions. While PRP induced a significant 2-fold increase in osteoblast migration, i-PRF demonstrated a 3-fold increase in migration when compared to control tissue-culture plastic and PRP. While no differences were observed for cell attachment, i-PRF induced a significantly higher proliferation rate at three and five days when compared to PRP. Furthermore, i-PRF induced significantly greater ALP staining at 7 days and alizarin red staining at 14 days. A significant increase in mRNA levels of ALP, Runx2 and osteocalcin, as well as immunofluorescent staining of osteocalcin was also observed in the i-PRF group when compared to PRP. In conclusion, the results from the present study favored the use of the naturally-formulated i-PRF when compared to traditional PRP with anti-coagulants. Further investigation into the direct role of fibrin and leukocytes contained within i-PRF are therefore warranted to better elucidate their positive role in i-PRF on tissue wound healing.
富血小板血浆 (PRP) 多年来一直被用作一种再生剂，能够利用血液来源的生长因子诱导各种组织的血管生成。尽管如此，主要与 PRP 中添加的抗凝剂相关的缺点已被证明会抑制伤口愈合过程。出于这些原因，最近开发了一种新型血小板浓缩物，利用较低的离心速度，不含添加剂。因此，本研究的目的是研究这种新型治疗方法（注射用富血小板纤维蛋白；i-PRF，100%天然，无添加剂）对成骨细胞行为的影响，并与传统 PRP 进行比较。将人原代成骨细胞与 i-PRF 或 PRP 共培养，并与对照组织培养塑料进行比较。研究了活/死测定、迁移测定以及细胞黏附和增殖测定。此外，通过碱性磷酸酶 (ALP)、茜素红和骨钙素染色以及编码 Runx2、ALP、胶原蛋白 1 和骨钙素的基因的实时 PCR 评估成骨细胞分化。结果表明，无论培养条件如何，所有细胞在整个研究期间的存活率都很高。虽然 PRP 诱导成骨细胞迁移显著增加了 2 倍，但与对照组织培养塑料和 PRP 相比，i-PRF 诱导的迁移增加了 3 倍。虽然细胞附着没有差异，但与 PRP 相比，i-PRF 在第 3 天和第 5 天诱导的增殖率显著更高。此外，i-PRF 在第 7 天诱导的 ALP 染色和第 14 天诱导的茜素红染色显著增加。与 PRP 相比，i-PRF 组的 ALP、Runx2 和骨钙素的 mRNA 水平以及骨钙素的免疫荧光染色也显著增加。总之，与含有抗凝剂的传统 PRP 相比，本研究结果更倾向于使用天然配方的 i-PRF。因此，有必要进一步研究 i-PRF 中纤维蛋白和白细胞的直接作用，以更好地阐明它们在 i-PRF 对组织伤口愈合中的积极作用。
